Nano-pesticide carrier O-Carboxymethyl chitosan is indigestible in Apis cerana cerana and affects intestinal flora

被引：4

作者：

Li, Sheng-nan ^{[1
]}

Wu, Hao ^{[1
]}

Hou, Rui-quan ^{[1
]}

Zhou, Jing-tong ^{[1
]}

Lin, Su-kun ^{[1
]}

Zhao, Kun-yu ^{[1
]}

Wang, Rui-fei ^{[1
]}

Huang, Su-qing ^{[2
]}

Zhang, Zhi-xiang ^{[1
]}

机构：

[1] South China Agr Univ, Minist Educ, Key Lab Nat Pesticide & Chem Biol, Guangzhou 510642, Peoples R China

[2] Zhongkai Univ Agr & Engn, Guangzhou 510225, Peoples R China

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2023年 / 885卷

关键词：

O-Carboxymethyl chitosan nanoparticles; Apis cerana cerana; Oxidative stress; Intestinal flora; NANOPARTICLES; RESPONSES; DELIVERY; EXPOSURE; CURCUMIN; MODEL;

D O I：

10.1016/j.scitotenv.2023.163769

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

O-Carboxymethyl chitosan nanoparticles (O-CMC-NPs), which are organic pesticide carriers, have excellent applica-tion potential. Exploring the effects of O-CMC-NPs on non-target organisms, such as Apis cerana cerana, is critical for their effective application; however, such studies are limited. This study investigated the stress response of A. cerana Fabricius after O-CMC-NPs ingestion. The administration of high O-CMC-NP concentrations enhanced the activities of antioxidant and detoxifying enzymes in A. cerana, with the activity of glutathione-S-transferase increasing by 54.43 %-64.33 % after one day. The transit of O-CMC-NPs into the A. cerana midgut resulted in their deposition and adherence to the intestinal wall, as they cluster and precipitate in acidic conditions. The population of Gillianella bacteria in the middle intestine was remarkably reduced after 6 d of administration of high O-CMC-NP concentrations. Contrastingly, the abundance of Bifidobacteria and Lactobacillus in the rectum significantly increased. These results in-dicate that the intake of high concentrations of O-CMC-NPs causes a stress response in A. cerana and affects the relative abundance of crucial intestinal flora, which may pose a potential risk to the colony. This implies that even nanomate-rials with favorable biocompatibility should be applied reasonably within a specific range to avoid adverse effects on the environment and non-target organisms in the context of large-scale research and promotion of nanomaterials.

引用

页数：8