Fermentation profile, aerobic stability, and microbial community dynamics of corn straw ensiled with Lactobacillus buchneri PC-C1 and Lactobacillus plantarum PC1-1

Corn straw is suitable for preservation as silage despite being neglected due to its varying chemical composition, yield, and pathogenic influence during ensiling. This study examined the effects of beneficial organic acid-producing lactic acid bacteria (LAB), including Lactobacillus buchneri (Lb), L. plantarum (Lp), or their combina-tion (LpLb), on fermentation profile, aerobic stability, and microbial community dynamics of corn straw har-vested at late maturity stage after 7d, 14d, 30d, and 60d of ensiling. Higher levels of beneficial organic acids, LAB counts, and crude protein (CP), and lower levels of pH and ammonia nitrogen were detected in LpLb-treated silages after 60d. Lactobacillus , Candida, and Issatchenkia abundances were higher (P < 0.05) in Lb and LpLb-treated corn straw silages after 30d and 60d ensiling. Additionally, the positive correlation between Lactoba-cillus, Lactococcus and Pediococcus, and the negative correlation with Acinetobacter in LpLb-treated silages after 60d emphasizes a potent interaction mechanism initiated by organic acid and composite metabolite production to reduce pathogenic microorganisms' growth. Also, a significant correlation between Lb and LpLb-treated si -lages with CP and neutral detergent fiber after 60d further highlights the synergistic effect of incorporating L. buchneri and L. plantarum for improved nutritional components of mature silages. The combination of L. buchneri and L. plantarum improved aerobic stability, fermentation quality, and bacterial community and reduced fungal population after 60d of ensiling, which are properties of well-preserved corn straw.