The subsurface middle Eocene - early Miocene succession in the N. El Faras - 1X Well, drilled in the Qattara Depression at the northern part of the Western Desert, Egypt (Southern Tethyan Province, STP), was studied for its ostracode content to infer changes in paleobathymetry as well as paleobiogeographic reconstructions. This succession is composed mainly of three lithostratigraphic units, as the upper part of the Apollonia Formation (middle to late Eocene), the Dabaa Formation (late Eocene and Oligocene) and the lower part of the Moghra Formation (early Miocene). The stratigraphical distribution, paleoenvironmental and paleobiogeographical significance of the ostracode faunas from the Oligocene interval of the study area are documented for the first time. Fifty seven ostracode species belonging to 36 genera were identified in this succession. Among them, two new species were described. The vertical distribution of these ostracode species enabled the recognition of seven local biozones. Qualitative and quantitative statistical analysis of ostracode associations revealed a depositional depth ranging from the inner to outer neritic setting. Considering the CONISS cluster analysis, six ecozones were established. During the middle and late Eocene, the paleobiogeographical affinities of the Egyptian ostracodes from N. El Faras-1Xx Well succession with that of the Southern Tethyan countries suggest a direct connection between Egypt and the countries of the Southern Tethyan Province. This connection facilitated the dispersal and exchange within this province. The low ostracode similarity between the STP and the Northern Tethyan Province (NTP) suggests a slight connection and exchange between them. The in common occurrence of these ostracodes with that of Somalia is attributed to the connection between the STP and Somalia via the Tethyan Seaway, which facilitated the dispersal and exchange of the middle Eocene ostracodes. The similarity between the Egyptian ostracodes (STP) and that of South America and the Caribbean Sub-Province indicates a direct connection between those provinces across the Gibraltar Strait and through the North Equatorial Oceanic Water Current during that time. During the Oligocene, the low ostracode similarity between the STP and the NTP may be attributed to a more or less restricted connection. Also, the low similarity indicates a continuation of the mild connection between the STP and the South America and Caribbean Sub-Province across the Gibraltar Strait and through the North Equatorial Current. During the early Miocene, the similarity of the recorded ostracodes of the STP with that of the NTP suggests a continuous connection between the two provinces. Also, there was a connection between the South America and Caribbean Sub-Province and the North American Province through the North Equatorial Current and the warm Gulf Stream within the Caribbean Sea.
