Development and Validation of UV-Spectrophotometric Method for Estimation of in Marketed Formulation and Nanoformulation

A quick, accurate, and cost-effective UV spectroscopy method was developed to estimate the Vinpocetine concentration in bulk, tablet dosage formulations and niosomes formulations, using a solvent ratio of (6:4) methanol: water. According to ICH guidelines, the proposed technique was validated and developed. In spite of parameters were validated by using UV/visible spectroscopy technique to analyze a spiked Vinpocetine solution. The wavelength at which the drug's maximum absorbance peak was obtained at 274 nm and the solvents used as methanol: water (6:4 w/v). The ethanol injection technique was used to prepare niosomes to analyze Vinpocetine in UV / visible spectrophotometric method. During the inter and intra-day studies, it was discovered that the developed UV technique was accurate, with % relative standard deviation ranging from 0.27 to 0.46 and 0.26 to 0.46, respectively. Vinpocetine overall recovery percentage was discovered to be between 98.42 to 99.82 %. LOQ and LOD were calculated to estimate the method's sensitivity, and they were observed to be 0.4565 mu g/ml and 0.1506 mu g/ ml, respectively. The estimation of Vinpocetine content in bulk form, marketed formulations and niosomes was achieved using the developed methodology. : A quick, accurate, and economical UV spectrophotometric method has been developed that estimates the vinpocetine concentration in bulk, tablet dosage formulations, and niosomes formulations.