Optical performance measurement methods for whole slide imaging devices employing a Bayer color imager

Whole slide imaging (WSI) is a widely used digitalization technique to record microscopic images of a whole-stained tissue sample on a glass slide. The US Food and Drug Administration (FDA) provides guidelines for methods used to evaluate various WSI devices. However, the designated evaluation methods for guidance are not always optimal. To this end, novel inspection methods are proposed, which fulfill the requirements of FDA guidance, and then deployed to evaluate the measurement accuracy of our laboratory-built WSI device that employs a 2D Bayer imager. The proposed methods demonstrably evaluate distortion, chromatic aberration, and field curvature with the required precision.& COPY; 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement