Cellulomonas xiejunii sp. nov., Cellulomonas chengniuliangii sp. nov. and Cellulomonas wangsupingiae sp. nov., three cellulolytic bacteria isolated from intestinal contents of Marmota himalayana

Six facultative anaerobic, Gram- stain- positive, oxidase- negative, rod- shaped bacteria (strains zg- B89T, zg-B12, zg-Y338T, zgY138, zg-Y908T and zg- Y766), were isolated from the intestinal contents of Marmota himalayana in Qinghai Province, PR China. The 16S rRNA gene sequence analysis showed that zg- B89T showed highest similarity to Cellulomonas iranensis NBRC 101100T (99.5 %), zg-Y338T to Cellulomonas cellasea DSM 20118T (98.7 %), and zg- Y908T to Cellulomonas flavigena DSM 20109T (99.0 %). Phylogenetic and phylogenomic analysis based on 16S rRNA gene and 881 core genes revealed that these six strains formed three separate clades in the genus Cellulomonas. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between these three novel species and all members of the genus Cellulomonas were below species thresholds (95-96 % for ANI and 70 % for dDDH). The DNA G+C contents of zg- B89T, zg-Y338T and zg- Y908T were 73.6, 72.9 and 74.5 %, respectively. Strains zg-B89T and zg- Y908T had anteiso- C15:0, C16: 0 and anteiso- C15: 1 A, and zg- Y338T had anteiso- C15:0, C16: 0 and iso- C16: 0 as the main fatty acids. All novel type strains had MK- 9 (H4) as the predominant respiratory quinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside as the major polar lipids, and rhamnose, ribose and glucose as the cell- wall sugars. The peptidoglycan amino acids of zg- B89T, zg-Y338T and zg- Y908T contained ornithine, alanine, glutamic acid and aspartic acid (except for zg- Y338T). Based on genotypic, phenotypic, phylogenetic and biochemical properties, the six uncharacterized strains represent three novel species in the genus Cellulomonas, for which the names Cellulomonas xiejunii sp. nov. (type strain zg- B89T=GDMCC 1.2821T=KCTC 49756T), Cellulomonas chengniuliangii sp. nov. (type strain zg-Y338T=GDMCC 1.2829T=KCTC 49754T) and Cellulomonas wangsupingiae sp. nov. (type strain zg- Y908T=GDMCC 1.2820T=KCTC