Continuously released Zn2+ in 3D-printed PLGA/S-TCP/Zn scaffolds for bone defect repair by improving osteoinductive and anti-inflammatory properties

被引:49
|
作者
Li, Chunxu [1 ]
Sun, Fengbo [2 ]
Tian, Jingjing [3 ]
Li, Jiahao [1 ]
Sun, Haidan [4 ,5 ]
Zhang, Yong [4 ,6 ]
Guo, Shigong [7 ]
Lin, Yuanhua [2 ]
Sun, Xiaodan [2 ]
Zhao, Yu [1 ]
机构
[1] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Orthoped, Beijing 100730, Peoples R China
[2] Tsinghua Univ, Sch Mat, State Key Lab Adv Ceram & Fine Proc, Beijing, Peoples R China
[3] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Med Sci Res Ctr, Beijing, Peoples R China
[4] Chinese Acad Med Sci, Inst Basic Med Sci, Core Facil Instrument, Beijing, Peoples R China
[5] Peking Union Med Coll, Sch Basic Med, Beijing, Peoples R China
[6] Chinese Acad Med Sci, Inst Basic Med Sci, State Key Lab Med Mol Biol, Beijing, Peoples R China
[7] Southmead Hosp, Dept Rehabil Med, Bristol, England
基金
中国国家自然科学基金;
关键词
3D printing; Zinc submicron particles; Osteoinductivity; Anti-inflammatory; Bone defect repair; IN-VITRO; MORPHOGENETIC PROTEIN-2; SUSTAINED-RELEASE; POROUS SCAFFOLDS; LUMBAR FUSION; STEM-CELLS; ZINC; REGENERATION; COMPOSITE; DIFFERENTIATION;
D O I
10.1016/j.bioactmat.2022.12.015
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Long-term nonunion of bone defects has always been a major problem in orthopedic treatment. Artificial bone graft materials such as Poly (lactic-co-glycolic acid)/S-tricalcium phosphate (PLGA/S-TCP) scaffolds are expected to solve this problem due to their suitable degradation rate and good osteoconductivity. However, insufficient mechanical properties, lack of osteoinductivity and infections after implanted limit its large-scale clinical application. Hence, we proposed a novel bone repair bioscaffold by adding zinc submicron particles to PLGA/ S-TCP using low temperature rapid prototyping 3D printing technology. We first screened the scaffolds with 1 wt % Zn that had good biocompatibility and could stably release a safe dose of zinc ions within 16 weeks to ensure long-term non-toxicity. As designed, the scaffold had a multi-level porous structure of biomimetic cancellous bone, and the Young's modulus (63.41 +/- 1.89 MPa) and compressive strength (2.887 +/- 0.025 MPa) of the scaffold were close to those of cancellous bone. In addition, after a series of in vitro and in vivo experiments, the scaffolds proved to have no adverse effects on the viability of BMSCs and promoted their adhesion and osteogenic differentiation, as well as exhibiting higher osteogenic and anti-inflammatory properties than PLGA/S-TCP scaffold without zinc particles. We also found that this osteogenic and anti-inflammatory effect might be related to Wnt/S-catenin, P38 MAPK and NFkB pathways. This study lay a foundation for the follow-up study of bone regeneration mechanism of Zn-containing biomaterials. We envision that this scaffold may become a new strategy for clinical treatment of bone defects.
引用
收藏
页码:361 / 375
页数:15
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