Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test

被引：1

作者：

Paz, Larissa Ferreira de Araujo ^{[1
]}

da Silva, Adalucia ^{[2
]}

da Silva, Hemilly Rayanne Ferreira ^{[2
]}

Cavalcanti, Milena Paiva ^{[2
]}

de Lima, Valeria Marcal Felix ^{[3
]}

Beltrao, Maria Rosario Oliveira da Cunha ^{[1
]}

Silva, Maria Beatriz Araujo ^{[1
]}

Neto, Osvaldo Pompilio de Melo ^{[2
]}

Medeiros, Zulma Maria ^{[1
,2
]}

dos Santos, Wagner Jose Tenorio ^{[2
]}

机构：

[1] Univ Pernambuco, Postgrad Program Hlth Sci, BR-50100010 Recife, Brazil

[2] Aggeu Magalhaes Inst Fiocruz, BR-50740465 Recife, Brazil

[3] Sao Paulo State Univ, Coll Vet Med, Dept Clin Surg & Anim Reprod, BR-16050680 Aracatuba, Brazil

来源：

VETERINARY SCIENCES | 2023年 / 10卷 / 10期

关键词：

Leishmania infantum; canine visceral leishmaniasis; DPP test; SERODIAGNOSIS; DOGS;

D O I：

10.3390/vetsci10100608

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Dogs are considered the major domestic reservoir for human visceral leishmaniasis, a serious disease caused by the Leishmania infantum parasite. Diagnosis of canine visceral leishmaniasis (CVL) is critical for disease control, with several methods currently available. Among the serological tests, the DPP rapid test and the EIE-LVC, more commonly used in Brazil, are associated with variable sensitivity and specificity. Research with novel recombinant proteins such as the ELISA with the recombinant chimeric protein Q5 may therefore improve the CVL diagnosis. This study aimed to evaluate the true diagnostic potential of Q5 in an ELISA assay using a large number of CVL-suspected sera (406) with a previous positive diagnosis based on the rapid DPP test. Sera from the DPP-positive dogs, also assessed with the EIE-LVC test, were compared with sera from healthy dogs (n = 46) and used for ELISA tests using the recombinant Q5. The resulting data as well as the correlation with the clinical signs and the environmental characteristics of the animals were analyzed using Medal and GraphPad Prism 8.0. Overall, similar levels of lower sensitivity (67-68%) were seen for both the commercial EIE-LVC test and the Q5 ELISA when all assessed sera were considered, but a much greater sensitivity (92%) was seen for those samples from symptomatic dogs only. In contrast, many negative results were observed for the DPP-positive sera from asymptomatic dogs or those with no clinical information available. A selection of those sera were tested yet again in new ELISA assays using a second batch of the recombinant Q5, purified under milder denaturing conditions, as well as using another recombinant protein (Lci13). The results reveal a higher-than-expected incidence of likely false-positive results for DPP, reinforcing the need for other recombinant proteins, such as the chimeric Q5, to be investigated as possible alternatives to the currently used CVL diagnostic methods.

引用

页数：12