Draft genome assemblies for two species of Escallonia (Escalloniales)

被引:0
|
作者
Chanderbali, Andre S. [1 ]
Dervinis, Christopher [2 ]
Anghel, Ioana G. [3 ]
Soltis, Douglas E. [1 ]
Soltis, Pamela S. [1 ]
Zapata, Felipe [4 ,5 ]
机构
[1] Univ Florida, Florida Museum Nat Hist, Gainesville, FL 32611 USA
[2] Univ Florida, Sch Forest Fisheries & Geomatics Sci, Gainesville, FL 32603 USA
[3] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA USA
[4] Univ Calif Los Angeles, Inst Environm & Sustainabil, Dept Ecol & Evolutionary Biol, Los Angeles, CA USA
[5] Univ Calif Los Angeles, Inst Environm & Sustainabil, Ctr Trop Res, Los Angeles, CA USA
来源
BMC GENOMIC DATA | 2024年 / 25卷 / 01期
关键词
Andes; Campanulidae; Escallonia rubra; Escallonia herrerae; Genomics; Oxford Nanopore Technology; DIVERSIFICATION;
D O I
10.1186/s12863-023-01186-7
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Objectives Escallonia (Escalloniaceae) belongs to the Escalloniales, a diverse clade of flowering plants with unclear placement in the tree of life. Escallonia species show impressive morphological and ecological diversity and are widely distributed across three hotspots of biodiversity in the Neotropics. To shed light on the genomic substrate of this radiation and the phylogenetic placement of Escalloniales as well as to generate useful data for comparative evolutionary genomics across flowering plants, we produced and annotated draft genomes for two species of Escallonia.Data description Genomic DNA from E. rubra and E. herrerae was sequenced with Oxford Nanopore sequencing chemistry, generating 3.4 and 12 million sequence reads with an average read length of 9.4 and 9.1 Kb (approximately 31 and 111 Gb of sequence data), respectively. In addition, we generated Illumina 100-bp paired-end short read data for E. rubra (approximately 75 Gb of sequence data). The Escallonia rubra genome was 566 Mb, with 3,233 contigs and an N50 of 285 Kb. The assembled genome for E. herrerae was 994 Mp, with 5,760 contigs and an N50 of 317 Kb. The genome sequences were annotated with 31,038 (E. rubra) and 47,905 (E. herrerea) protein-coding gene models supported by transcriptome/protein evidence and/or Pfam domain content. BUSCO assessments indicated completeness levels of approximately 98% for the genome assemblies and 88% for the genome annotations.
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页数:4
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