Expand and Sensitize: Guanidine-Functionalized Exopolysaccharide Nanoparticles Cause Bacterial Cell Expansion and Antibiotic Sensitization

被引：14

作者：

Li, Chengcheng ^{[1
,2
]}

Jia, Hao-Ran ^{[3
]}

Seidi, Farzad ^{[1
,2
]}

Shi, Xiaotong ^{[1
,2
]}

Gu, Ruihan ^{[1
,2
]}

Guo, Yuxin ^{[3
]}

Liu, Yi ^{[3
]}

Zhu, Ya-Xuan ^{[3
]}

Wu, Fu-Gen ^{[3
]}

Xiao, Huining ^{[4
]}

机构：

[1] Nanjing Forestry Univ, Int Innovat Ctr Forest Chem & Mat, Nanjing 210037, Peoples R China

[2] Nanjing Forestry Univ, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat Fo, Nanjing 210037, Peoples R China

[3] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Digital Med Engn, Jiangsu Key Lab Biomat & Devices, Nanjing 210096, Peoples R China

[4] Univ New Brunswick, Dept Chem Engn, Fredericton, NB E3B 5A3, Canada

来源：

ADVANCED FUNCTIONAL MATERIALS | 2023年 / 33卷 / 51期

基金：

中国国家自然科学基金; 加拿大自然科学与工程研究理事会;

关键词：

antibacterial; antibiofilm; bacterial cell expansion; RNA degradation; wound healing; STAPHYLOCOCCUS-AUREUS; ANTIBACTERIAL ACTION; BIOFILM FORMATION; RESISTANCE; FLUORESCENCE; STRESS;

D O I：

10.1002/adfm.202305977

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Conventional antibacterial agents and mechanisms are frequently observed to be ineffective due to the evolution of bacteria to the strains with stronger antibiotic resistance, and hence developing alternative antibacterial materials and mechanisms is urgently needed. Here, guanidine-functionalized exopolysaccharide (EPS) nanoparticles (termed EPGNs) with durable antibacterial and antibiofilm activities are developed. Very interestingly, the EPGNs obtained by the reaction of EPS, epichlorohydrin, and polyhexamethylene guanidine hydrochloride exhibit an unconventional antibacterial mechanism, i.e., they can induce substantial bacterial cell expansion by upregulating the SulA and DicB proteins that are responsible for cell division inhibition, along with the increase of reactive oxygen species production, bacterial cell surface disruption, and bacterial ribosomal RNA degradation. The transcriptome analysis reveals that EPGNs can hinder cell motility, induce loss of cell integrity, decrease the resistance of bacteria to oxidative stress, and finally lead to cell death. Moreover, EPGNs can effectively accelerate the bacteria-infected wound healing. This work provides the first example that nanomaterials can cause bacterial cell expansion by affecting intracellular structures and inhibiting cell division, and it may inspire other researchers to investigate the effect of antibacterial materials on the change of bacterial volume and design unconventional antibacterial materials/strategies. Guanidine-functionalized exopolysaccharide nanoparticles exhibit an unconventional antibacterial mechanism, i.e., they can induce substantial bacterial cell expansion by upregulating the SulA and DicB proteins that are responsible for cell division inhibition, along with the increase of reactive oxygen species production, bacterial cell surface disruption, and bacterial ribosomal RNA degradation. Such a bacterial expansion mechanism renders the nanoparticles an efficacious antibiotic adjuvant.image

引用

页数：16