Piceatannol Alleviates Deoxynivalenol-Induced Damage in Intestinal Epithelial Cells via Inhibition of the NF-κB Pathway

被引:1
|
作者
Zhu, Min [1 ,2 ]
Lu, En-Qing [1 ,2 ]
Fang, Yong-Xia [1 ,2 ]
Liu, Guo-Wei [1 ,2 ]
Cheng, Yu-Jie [1 ,2 ]
Huang, Ke [1 ,2 ]
Xu, E. [1 ,2 ]
Zhang, Yi-Yu [1 ,2 ]
Wang, Xiao-Jing [3 ]
机构
[1] Guizhou Univ, Coll Anim Sci, Key Lab Anim Genet Breeding & Reprod Plateau Mt Re, Minist Educ, Guiyang 550025, Peoples R China
[2] Guizhou Univ, Inst Anim Nutr & Feed Sci, Guiyang 550025, Peoples R China
[3] Guizhou Univ, Inst Agrobioengn, Key Lab Plant Resource Conservat & Germplasm Innov, Minist Educ, Guiyang 550025, Peoples R China
来源
MOLECULES | 2024年 / 29卷 / 04期
基金
中国国家自然科学基金;
关键词
piceatannol; deoxynivalenol; oxidative stress; NF-kappa B pathway; intestinal barrier; TIGHT JUNCTION PROTEINS; OXIDATIVE STRESS; BARRIER FUNCTION; DON; INFLAMMATION; EXPRESSION; MYCOTOXIN; INGESTION; APOPTOSIS; TOXICITY;
D O I
10.3390/molecules29040855
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Deoxynivalenol (DON) is a common mycotoxin that is widely found in various foods and feeds, posing a potential threat to human and animal health. This study aimed to investigate the protective effect of the natural polyphenol piceatannol (PIC) against DON-induced damage in porcine intestinal epithelial cells (IPEC-J2 cells) and the underlying mechanism. The results showed that PIC promotes IPEC-J2 cell proliferation in a dose-dependent manner. Moreover, it not only significantly relieved DON-induced decreases in cell viability and proliferation but also reduced intracellular reactive oxygen species (ROS) production. Further studies demonstrated that PIC alleviated DON-induced oxidative stress damage by increasing the protein expression levels of the antioxidant factors NAD(P)H quinone oxidoreductase-1 (NQO1) and glutamate-cysteine ligase modifier subunit (GCLM), and the mRNA expression of catalase (CAT), Superoxide Dismutase 1 (SOD1), peroxiredoxin 3 (PRX3), and glutathione S-transferase alpha 4 (GST alpha 4). In addition, PIC inhibited the activation of the nuclear factor-B (NF-kappa B) pathway, downregulated the mRNA expression of interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-alpha) to attenuate DON-induced inflammatory responses, and further mitigated DON-induced cellular intestinal barrier injury by regulating the protein expression of Occludin. These findings indicated that PIC had a significant protective effect against DON-induced damage. This study provides more understanding to support PIC as a feed additive for pig production.
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页数:13
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