Noradrenaline and serotonin-dependent sensitization of MSCs to noradrenaline

被引：0
|
作者：
Chechekhin, Vadim I. [1 ]
Kulebyakin, Konstantin Yu. [1 ]
Kalinina, Natalia I. [1 ]
Tyurin-Kuzmin, Pyotr A. [1 ]
机构：
[1] Lomonosov Moscow State Univ, Fac Med, Dept Biochem & Regenerat Biomed, Moscow 119991, Russia
来源：
METHODSX | 2024年 / 12卷
基金：
俄罗斯科学基金会;
关键词：
Noradrenaline; Serotonin; Mesenchymal stem cells; Adipose tissue; Obesity; Obesity-associated hypertension;
D O I：
10.1016/j.mex.2024.102587
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Stem and progenitor cells are characterized by peculiar mechanisms of hormonal regulation. Here we describe a protocol of analysis of hormonal cross -talk in adipose tissue derived multipotent mesenchymal stem cells (MSCs). Specifically, cells were treated by a "sensitizing " hormone/neuromediator followed by the measurement of cellular Ca2 + response to the "readout " hormone after various time intervals. This protocol was successfully used in studies demonstrating a permissive effect of noradrenaline and 5 -HT on MSCs sensitivity to noradrenaline, which is a predictive marker of the development of obesity -associated arterial hypertension.
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