Improving effect of disaccharides and maltodextrin on preparation of egg yolk powder by microwave-assisted freeze-drying: Functional properties, structural properties, and retention rate of active IgY br

被引:21
|
作者
Wang, Zhe [1 ]
Duan, Xu [1 ]
Ren, Guangyue [1 ]
Guo, Jingfang [1 ]
Ji, Jiangtao [2 ]
Xu, Yiming [1 ]
Cao, Weiwei [1 ]
Li, Linlin [1 ]
Zhao, Mengyue [1 ]
Ang, Yuan [1 ]
Jin, Xin [1 ]
Bhandari, Bhesh [3 ]
Zhu, Lewen [1 ]
Zhang, Yan [1 ]
机构
[1] Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471000, Peoples R China
[2] Henan Univ Sci & Technol, Collage Agr Equipment Engn, Luoyang 471023, Peoples R China
[3] Univ Queensland, Sch Agr & Food Sci, Brisbane, Qld 4072, Australia
基金
中国国家自然科学基金;
关键词
MFD; EYP; Solubility; Protein structure; Confocal laser scanning microscopy; IgY; NUCLEAR-MAGNETIC-RESONANCE; PHYSICOCHEMICAL PROPERTIES; PHYSICAL-PROPERTIES; TEMPERATURE; ULTRASOUND; STABILITY; PRESSURE; PROTEINS; FRACTION;
D O I
10.1016/j.foodchem.2022.134626
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The preparation of egg yolk powder (EYP) with excellent solubility and high retention of active IgY is of great significance for increasing the added value and promoting the application of EYP. A new method of preparing EYP by microwave-assisted freeze-drying (MFD) was researched. Confocal laser scanning microscopy results demonstrated that the supplementation of excipients (sucrose, trehalose, and maltodextrin) could inhibit lipoproteins aggregation in egg yolk induced by freezing. Scanning electron microscopy indicated that drying further damaged the structure of lipoproteins in EYP, leading to lipid separation from it. FTIR and fluorescence spectra confirmed this finding, indicating that excipients enhance protein stability. Compared with conventional freezedrying (FD), EYP prepared by MFD, particularly that containing excipients, had higher solubility (63 g/100 g), active antibody retention rate and shorter drying time. Therefore, excipients can significantly improve the solubility and stability of EYP and the retention rate of active IgY.
引用
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页数:11
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