Optical coherence microscopy with a split-spectrum image reconstruction method for temporal-dynamics contrast-based imaging of intracellular motility

被引：1

作者：

Kang, Yong Guk ^{[1
]}

Canoy, Raymart Jay E. ^{[2
]}

Jang, Yongjun ^{[3
]}

Santos, Ana Rita M. P. ^{[3
]}

Son, Inwoo ^{[3
]}

Kim, Beop-Min ^{[1
,4
]}

Park, Yongdoo ^{[3
]}

机构：

[1] Korea Univ, BK21 Four Inst Precis Publ Hlth, Seoul 02841, South Korea

[2] Korea Univ, Coll Engn, Dept Biomicro Syst Technol, Seoul 02841, South Korea

[3] Korea Univ, Coll Med, Dept Biomed Sci, Seoul 02841, South Korea

[4] Korea Univ, Coll Hlth Sci, Dept Biomed Engn, Seoul 02841, South Korea

来源：

BIOMEDICAL OPTICS EXPRESS | 2023年 / 14卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

ULTRAHIGH-RESOLUTION; BLOOD-FLOW; TOMOGRAPHY; POLARIZATION; KINASE; SPEED;

D O I：

10.1364/BOE.478264

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Biomedical researchers use optical coherence microscopy (OCM) for its high resolution in real-time label-free tomographic imaging. However, OCM lacks bioactivity-related functional contrast. We developed an OCM system that can measure changes in intracellular motility (indicating cellular process states) via pixel-wise calculations of intensity fluctuations from metabolic activity of intracellular components. To reduce image noise, the source spectrum is split into five using Gaussian windows with 50% of the full bandwidth. The technique verified that F-actin fiber inhibition by Y-27632 reduces intracellular motility. This finding could be used to search for other intracellular-motility-associated therapeutic strategies for cardiovascular diseases.

引用

页码：577 / 592

页数：16