Effects of Microencapsulated Blend of Organic Acids and Pure Botanicals on the Ruminal Microbiota in an In Vitro Dual-Flow Continuous Culture System

被引:1
|
作者
Lobo, Richard R. [1 ]
Roesch, Luiz Fernando Wurdig [2 ]
Grilli, Ester [3 ,4 ]
Faciola, Antonio P. [1 ]
机构
[1] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA
[2] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA
[3] Univ Bologna, Dipartimento Sci Med Veterinarie, I-40064 Bologna, Italy
[4] Vetagro SpA, I-42124 Reggio Emilia, Italy
来源
FERMENTATION-BASEL | 2023年 / 9卷 / 08期
关键词
citric acid; liquid-associated microenvironment; solid-associated microenvironment; sorbic acid; thymol; vanillin; RUMEN;
D O I
10.3390/fermentation9080730
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The objective of the study was to evaluate the effects of dietary supplementation of a microencapsulated blend of organic acids and pure botanicals (mOAPBs) on the solid- and liquid-associated microenvironment (SAM and LAM, respectively) of the ruminal microbiome using an in vitro dual-flow continuous culture system. Ruminal content was incubated in eight fermenters and the basal diet was supplemented with increasing levels of mOAPBs (0; 0.12; 0.24; or 0.36% DM) which contained 55.6% hydrogenated and refined palm oil, 25% citric acid, 16.7% sorbic acid, 1.7% thymol, and 1% vanillin. All diets had a similar nutritional composition (16.1 CP, 30.9 NDF, and 32.0 starch, % DM basis). After 7 days of adaptation, a pooled sample across the days was collected in each period for identification of the microbiome of SAM and LAM. There was no effect of mOAPB on alpha-, beta-diversity, and microbial abundance. The SAM had a greater bacterial diversity and the principal component analysis demonstrated that it had a divergent bacterial profile from LAM. Additionally, SAM had an increased abundance of carbohydrate-degrading microorganisms. In summary, mOAPBs did not modulate the ruminal microbiome. The microenvironment microbiome of solid- and liquid-associated microenvironments were different, with SAM having a greater carbohydrate-degrading microorganism population.
引用
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页数:10
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