In Vitro Isolation of Treponema pallidum subsp. pallidum From Fresh and Frozen Needle Aspirates of Primary Experimental Syphilis Lesions

被引：3
|
作者：
Tantalo, Lauren C. [1 ]
Molini, Barbara J. [1 ]
Bose, Mahashweta [1 ]
Klausner, Jeffrey D. [2 ]
Giacani, Lorenzo [1 ,3 ,4 ]
机构：
[1] Univ Washington, Dept Med, Div Allergy & Infect Dis, Seattle, WA USA
[2] Univ Southern Calif, Keck Sch Med, Los Angeles, CA USA
[3] Univ Washington, Dept Global Hlth, Seattle, WA USA
[4] Univ Washington, Harborview Med Ctr, 325 9th Ave, Seattle, WA 98104 USA
来源：
SEXUALLY TRANSMITTED DISEASES | 2023年 / 50卷 / 03期
基金：
美国国家卫生研究院;
关键词：
AMNIOTIC-FLUID; FETAL;
D O I：
10.1097/OLQ.0000000000001739
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Isolation of Treponema pallidum subsp. pallidum strains still relies on rabbit intratesticular inoculation of clinical samples. In this article, we report an alternative isolation approach based on the inoculation of fresh and frozen needle aspirates of primary experimental lesions into culture plates suitable for in vitro propagation of the syphilis agent.
引用
收藏
页码：180 / 183
页数：4
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