The effect of the properties of cell nucleus and underlying substrate on the response of finite element models of astrocytes undergoing mechanical stimulations

Astrocyte cells play a critical role in the mechanical behaviour of the brain tissue; hence understanding the properties of Astrocytes is a big step toward understanding brain diseases and abnormalities. Conventionally, atomic force microscopy (AFM) has been used as one of the most powerful tools to characterize the mechanical properties of cells. However, due to the complexities of experimental work and the complex behaviour of living cells, the finite element method (FEM) is commonly used to estimate the cells' response to mechanical stimulations. In this study, we developed a finite element model of the Astrocyte cells to investigate the effect of two key parameters that could affect the response of the cell to mechanical loading; the properties of the underlying substrate and the nucleus. In this regard, the cells were placed on two different substrates in terms of thickness and stiffness (gel and glass) with varying properties of the nucleus. The main achievement of this study was to develop an insight to investigate the response of the Astrocytes to mechanical loading for future studies, both experimentally and computationally.