Gold Nanoparticles Mineralized by Peptide Liquid Crystals with Dual-Functional Enzyme-like Activities: An Automatic Membrane Reactor for Glucose Detection

Thedevelopment of stable multifunctional enzyme mimics with tandemcatalytic effects provides a great opportunity to construct economicaland convenient bioassays. Inspired by biomineralization, in this workself-assembled N-(9-fluorenylmethoxycarbonyl)-protectedtripeptide (Fmoc-FWK-NH2) liquid crystals were used astemplates to in situ mineralize Au nanoparticles (AuNPs), and thena dual-functional enzyme-mimicking membrane reactor based on AuNPsand peptide-based hybrids was constructed. AuNPs with a uniform particlesize and good dispersion were in situ reduced on the surface of thepeptide liquid crystal due to the reduction of the indole group onthe tryptophan residue, which exhibited excellent peroxidase-likeand glucose oxidase-like activities simultaneously. Meanwhile, theoriented nanofibers aggregated into a three-dimensional network, whichwas further immobilized on the mixed cellulose membrane to form amembrane reactor. A biosensor was made to realize fast, low-cost,and automatic detection for glucose. This work represents a promisingplatform for the design and construction of novel multifunctionalmaterials based on the biomineralization strategy.