Marinomonas transparens sp. nov. and Marinomonas sargassi sp. nov., isolated from marine alga

Two Gram-stain-negative, rod-shaped, non-spore-forming, strictly aerobic, motile bacteria with a single polar flagellum, designated strains C1424(T) and C2222(T), were isolated from marine alga collected from the sea shore at Yantai, PR China. Strain C1424(T) grew at 4-37 degrees C and in the presence of 1-9 % (w/v) NaCl, while strain C2222(T) grew at 4-32 degrees C with 1-6 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences and concatenated amino acid sequences of 120 ubiquitous single-copy proteins showed that both strains C1424(T) and C2222(T) belonged to the genus Marinomonas, showing highest 16S rRNA gene sequence similarities to the type strains of Marinomonas primoryensis (98.1 %) and Marinomonas dokdonensis (98.1 %), respectively. The major fatty acids of the two strains were C-18 : 1 omega 6c and/or C-18 : 1 omega 7c, C-16 : 1 omega 6c and/or C-16 : 1 omega 7c and C-16 : 0, their predominant polar lipids were phosphatidylethanolamine and phosphatidylglycerol, and their sole respiratory quinone was Q8. On the basis of polyphasic analyses, strains C1424(T) and C2222(T) are considered to represent two novel species within the genus Marinomonas, for which the names Marinomonas transparens sp. nov. and Marinomonas sargassi sp. nov. are proposed. The type strains are C1424(T) (=KCTC 72119(T)=MCCC 1K03601(T)) and C2222(T) (=KCTC 72120(T)=MCCC 1K03602(T)), respectively.