Aerococcus viridans Phage Lysin AVPL Had Lytic Activity against Streptococcus suis in a Mouse Bacteremia Model

被引:5
|
作者
Xi, Hengyu [1 ]
Fu, Yao [1 ]
Chen, Chong [1 ]
Feng, Xin [1 ]
Han, Wenyu [1 ,2 ]
Gu, Jingmin [1 ,2 ]
Ji, Yalu [1 ]
机构
[1] Jilin Univ, Inst Zoonosis, State Key Lab Diag & Treatment Severe Zoonot Infec, Coll Vet Med,Key Lab Zoonosis Res,Minist Educ, Changchun 130062, Peoples R China
[2] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China
基金
中国国家自然科学基金;
关键词
Streptococcus suis; Aerococcus viridans; biofilm; bacteremia; phage lysin; BACTERIOPHAGE LYSIN; BIOFILM FORMATION; INFECTION; MICE; MENINGITIS; ENDOLYSINS; VIRULENCE; PROTECTS; CAPSULE;
D O I
10.3390/ijms242316670
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Streptococcus suis (S. suis) is a swine pathogen that can cause sepsis, meningitis, endocarditis, and other infectious diseases; it is also a zoonotic pathogen that has caused a global surge in fatal human infections. The widespread prevalence of multidrug-resistant S. suis strains and the decline in novel antibiotic candidates have necessitated the development of alternative antimicrobial agents. In this study, AVPL, the Aerococcus viridans (A. viridans) phage lysin, was found to exhibit efficient bactericidal activity and broad lytic activity against multiple serotypes of S. suis. A final concentration of 300 mu g/mL AVPL reduced S. suis counts by 4-4.5 log10 within 1 h in vitro. Importantly, AVPL effectively inhibited 48 h S. suis biofilm formation and disrupted preformed biofilms. In a mouse model, 300 mu g/mouse AVPL protected 100% of mice from infection following the administration of lethal doses of multidrug-resistant S. suis type 2 (SS2) strain SC19, reduced the bacterial load in different organs, and effectively alleviated inflammation and histopathological damage in infected mice. These data suggest that AVPL is a valuable candidate antimicrobial agent for treating S. suis infections.
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页数:14
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