Green Synthesis of Silver Nanoparticles Using Aqueous Extract of Vachellia xanthophloea and Their Potential Use for Antibacterial and Sensing of Mercury Ions

The use of silver nanoparticles (Ag-NPs) in different fields such as agriculture, medicine, and environment has recently increased. As a result, studies on their synthesis and applications have gained attention. In the present study, the synthesis of Ag-NPs using cheap, renewable, and environmentally friendly phytochemicals extracted from Vachellia xanthophloea leaves is reported. Additionally, potential uses of the nanoparticles against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria and for sensing of mercury ions from aqueous solutions were investigated. The findings showed that the formation of nanoparticles was associated with a color change from green to brown with a UV-Vis maximal absorbance peak at 420 nm due to surface plasmon resonance on Ag-NPs. The synthesized Ag-NPs showed effective antibacterial effects against both gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria. The antibacterial effectiveness as indicated by the minimum inhibitory concentration (MIC) was higher for Staphylococcus aureus (0.04 mg/mL) compared to E. coli (0.33 mg/mL). Similarly, the minimum bactericidal concentration (MBC) values were 0.04 and 0.66 mg/mL for S. aureus and E. coli, respectively. The nanoparticles were also sensitive to metal ions (Hg2+, Ba2+, Mg2+, Zn2+, Cu2+, Ni2+, Co2+, and Fe2+) and showed a remarkable selectivity to mercury. The potential use of the nanoparticles for sensing of mercury ions from real water samples of ground and tap waters produced satisfactory results. The limit of detection (LOD) and limit of quantification (LQD) for sensing of Hg2+ were determined to be 22.2 & mu;M and 73.9 & mu;M, respectively. The findings from the current study indicate that Ag-NPs prepared using Vachellia xanthophloea leaves aqueous extract could serve as effective an antibacterial agent and a sensor of mercury ions for aqueous samples.