Species in genus Nannochloropsis, especially N. oceanica and N. gaditana, have been evolving as the model microalgae for both application and theory studies. The position effect of genome integration, the carrying capability limitation of integrative vectors and the instability of non-integrative vectors have hindered Nannochloropsis genetic modification with concatenate genes and extremely long DNA fragments. The molecular tools including genetic transformation, homologous recombination, gene edition, gene stacking and episome vectors for transient gene expression and diverse reporters and selection markers have been rapidly developing in Nannochloropsis species. The construction of animal and plant artificial chromosomes with "top down" strategy has set fine examples for the construction of Nannochloropsis artificial chromosomes (NannoACs). It seems that the methods and materials to set the foundation for constructing NannoACs are at hands. In this review, we outlined the current status of transgenes in Nannochloropsis species, summarized the limitations of both integrative and non-integrative vectors, and proposed a tentative approach to construct NannoACs by doubling and stabilizing the genome first, and then truncating the natural chromosomes. NannoACs once constructed will facilitate transferring the desired traits and concatenate genes into Nannochloropsis genetic backgrounds, thus contributing towards its genetic improvement and synthetic biological studies.
机构:
College of Marine Life Sciences, Ocean University of ChinaCollege of Marine Life Sciences, Ocean University of China
Li GUO
Guanpin YANG
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机构:
College of Marine Life Sciences, Ocean University of China
Institute of Evolution and Marine Biodiversity, Ocean University of China
Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of ChinaCollege of Marine Life Sciences, Ocean University of China
机构:
PSL Res Univ, Inst Curie, CNRS, UMR 144, 26 Rue Ulm, F-75005 Paris, FrancePSL Res Univ, Inst Curie, CNRS, UMR 144, 26 Rue Ulm, F-75005 Paris, France
Fachinetti, Daniele
Masumoto, Hiroshi
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Kazusa DNA Res Inst, Dept Frontier Res & Dev, Lab Chromosome Engn, 2-6-7 Kazusa Kamatari, Kisarazu 2920818, JapanPSL Res Univ, Inst Curie, CNRS, UMR 144, 26 Rue Ulm, F-75005 Paris, France
Masumoto, Hiroshi
Kouprina, Natalay
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NCI, Genome Struct & Funct Grp, Dev Therapeut Branch, Ctr Canc Res,NIH, Bethesda, MD 20892 USAPSL Res Univ, Inst Curie, CNRS, UMR 144, 26 Rue Ulm, F-75005 Paris, France