Light recovery after maize harvesting promotes soybean flowering in a maize-soybean relay strip intercropping system

Moving from sole cropping to intercropping is a transformative change in agriculture, contributing to yield. Soybeans adapt to light conditions in intercropping by adjusting the onset of reproduction and the inflorescence architecture to optimize reproductive success. Maize-soybean strip intercropping (MS), maize-soybean relay strip intercropping (IS), and sole soybean (SS) systems are typical soybean planting systems with significant differences in light environments during growth periods. To elucidate the effect of changes in the light environment on soybean flowering processes and provide a theoretical basis for selecting suitable varieties in various planting systems to improve yields, field experiments combining planting systems (IS, MS, and SS) and soybean varieties (GQ8, GX7, ND25, and NN996) were conducted in 2021 and 2022. Results showed that growth recovery in the IS resulted in a balance in the expression of TERMINAL FLOWER 1 (TFL1) and FLOWERING LOCUS T (FT) in the meristematic tissues of soybeans, which promoted the formation of new branches or flowers. IS prolonged the flowering time (2-7 days) and increased the number of forming flowers compared with SS (93.0 and 169%) and MS (67.3 and 103.3%) at the later soybean flowering stage. The higher carbon and nitrogen content in the middle and bottom canopies of soybean contributed to decreased flower abscission by 26.7 and 30.2%, respectively, compared with SS. Canopy light environment recovery promoted branch and flower formation and transformation of flowers into pods with lower flower-pod abscission, which contributed to elevating soybean yields in late-maturing and multibranching varieties (ND25) in IS. Differences in light conditions under various planting patterns lead to distinct flowering characteristics in soybeans. IS, maize-soybean relay strip intercropping system; MS, maize-soybean strip intercropping system; SS, sole soybean planting. GQ8, Gongqiu8; GX7, Guixia7; ND25, Nabdou25; NN996, Nannong996. imageVariations in shading environments across different planting systems impact soybean flowering and pod-setting, with post-maize harvest light recovery enhancing soybean flowering through increased expression of GmFT and GmLFY and promoting carbohydrate supply within the maize-soybean relay strip intercropping system. Our results offer new insights into the response and adaptability of soybean flowering to shading environments, laying a theoretical foundation for achieving high yields of soybeans under different planting systems.