First molecular detection of Mycoplasma ovis in horses from Brazil

被引：3

作者：

Kakimori, Monica T. A. ^{[1
,2
]}

Barros, Luiz D. ^{[2
]}

Collere, Flavia C. M. ^{[1
]}

Ferrari, Larissa D. R. ^{[1
]}

de Matos, Aldair ^{[2
]}

Lucas, Juliana, I ^{[3
]}

Coradi, Vanessa S. ^{[1
]}

Mongruel, Anna C. B. ^{[4
]}

Aguiar, Daniel M. ^{[5
]}

Machado, Rosangela Z. ^{[4
]}

Andre, Marcos R. ^{[4
]}

Vieira, Thallitha S. W. J. ^{[1
]}

Vieira, Rafael F. C. ^{[1
,6
]}

机构：

[1] Univ Fed Parana, Dept Vet Med, Vector Borne Dis Lab, Rua Funcionarios,Campus Agr 1540, BR-80035050 Curitiba, PR, Brazil

[2] Univ Estadual Londrina, Dept Prevent Vet Med, Londrina, Parana, Brazil

[3] Univ Fed Uberlandia, Dept Immunol Microbiol & Parasitol, Lab Immunoparasitol, Uberlandia, MG, Brazil

[4] Univ Estadual Paulista FCAV UNESP, Fac Ciencias Agr & Vet, Lab Imunoparasitol, Dept Patol Teriogenol & Saude Unica, Jaboticabal, Brazil

[5] Univ Fed Mato Grosso, Lab Virol & Rickettsioses, Cuiaba, Mato Grosso, Brazil

[6] Ohio State Univ, Global Hlth Initiat GOHi 1, Columbus, OH 43210 USA

来源：

ACTA TROPICA | 2023年 / 237卷

基金：

巴西圣保罗研究基金会;

关键词：

Babesia caballi; Hemotropic mycoplasma; Theileria equi; Dermacentor nitens; Ehrlichia sp; SAO-PAULO STATE; THEILERIA-EQUI; RURAL SETTLEMENT; BABESIA-CABALLI; PARANA STATE; EHRLICHIA; PREVALENCE; INFECTION; DOGS; TICKS;

D O I：

10.1016/j.actatropica.2022.106697

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

This study aimed to determine the occurrence of hemoplasmas and tick-borne pathogens (TBP) (Theileria equi, Babesia caballi, and Ehrlichia sp.) in horses and ticks' salivary glands, and determine the factors associated with exposure/infection in a rural settlement in southern Brazil. Blood samples from 22 horses were screened for anti -T. equi and anti-Ehrlichia sp. antibodies by an indirect fluorescent antibody test (IFAT) assays. Samples were also tested by PCR assays for T. equi and B. caballi (18S rRNA and rap-1 genes, respectively), hemoplasmas (16S rRNA gene), and Ehrlichia sp. (dsb gene). Ticks were removed from the animals (inspection) and the environment (flannel trawling and dry ice traps), and morphologically identified. Additionally, salivary glands DNA was extracted from 28 adult ticks infesting the animals and four nymphs from the environment, and further screened for Ehrlichia sp. and hemotropic Mycoplasma sp. Anti -T. equi and anti-Ehrlichia sp. antibodies were detected in 40.91% (nine/22; 95% CI: 23.26-61.27) and 31.81% (seven/22; 95% CI: 16.36-52.68) horses, respectively. Theileria equi, B. caballi, and hemotropic Mycoplasma sp. DNA was detected in 59.09% (13/22), 4.55% (one/22), and 50% (11/22) horses, respectively. All horses tested negative in the PCR for Ehrlichia sp. All sequences showed >= 99% identity with multiple T. equi, B. caballi, and Mycoplasma ovis sequences deposited in GenBank database. Adult ticks were identified as Dermacentor nitens (44/47; 93.62%) and Rhipicephalus microplus (three/47; 6.38%). Ticks' salivary glands were negative for Ehrlichia sp., while 39.29% from adults (11/28) and 50% from nymphs (two/four) from the environment were positive for hemotropic Mycoplasma sp. This is the first report of M. ovis infection in horses from Brazil and the first detection of hemoplasma DNA in salivary glands of D. nitens and R. microplus ticks. Further studies are needed to elucidate the vector competence of ticks to transmit hemoplasmas.

引用

页数：9

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