Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women

被引：1
|
作者：
Krysta-Matter, A. E. [1 ,2 ]
Riepsamen, A. H. [1 ]
Lien, S. [1 ]
Wong, W. Y. T. [1 ]
Richani, D. [1 ]
Kilani, S. [1 ,2 ]
Harrison, C. A. [3 ]
Mallitt, K. A. [1 ,4 ]
Ledger, W. L. [1 ,2 ]
Robertson, D. M. [1 ]
Gilchrist, R. B. [1 ]
机构：
[1] Univ New South Wales, Fertil & Res Ctr, Sch Clin Med, Discipline Womens Hlth, Kensington, NSW 2052, Australia
[2] IVF Australia, Virtus Hlth, Alexandria, NSW 2035, Australia
[3] Monash Univ, Monash Biomed Discovery Inst, Clayton, Vic 3800, Australia
[4] Univ New South Wales, Ctr Big Data Res Hlth, Sydney, NSW 2052, Australia
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2023年 / 578卷
基金：
英国医学研究理事会;
关键词：
Oocyte; Cumulus cells; Bone morphogenetic protein 15; Growth differentiating factor 9; Cumulin; GROWTH-DIFFERENTIATION FACTOR-9; MORPHOGENETIC PROTEIN 15; OOCYTE-SECRETED FACTORS; INCREASED OVULATION RATE; GRANULOSA-CELLS; HUMAN BMP-15; EXPRESSION; MUTATIONS; MOUSE; VARIANTS;
D O I：
10.1016/j.mce.2023.112049
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are oocyte-specific paracrine factors which regulate ovarian cumulus cell (CC) functions. This study aimed to investigate if BMP15 and GDF9 bound to CCs can be characterized, quantified, and show an association with IVF outcomes in infertile women. BMP15 and GDF9 ELISAs were validated and applied to discarded CC extracts. Pooled CCs from individual patients were collected from 120 (cohort 1; BMP15 only) and 81 infertility patients (cohort 2; BMP15 and GDF9) undergoing superovulation. BMP15 and GDF9 levels expressed per CC DNA were correlated with maternal age, clinical and embryology data. Total BMP15 and GDF9 were highly correlated with each other (r = 0.9, p < 0.001). The GDF9:BMP15 ratio was unrelated to oocyte number or age. BMP15/CC DNA and GDF9/CC DNA were unaffected by the type of superovulation and were not related to oocyte/embryo outcomes.
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页数：11
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