Precisely Constructing Renal-Clearable and LAP-Activatable Ratiometric Molecular Probes for Early Diagnosis of Acute and Chronic Kidney Injury Via Optimizing Asymmetric DPP Dyes

被引：22

作者：

Wang, Nannan ^{[1
,3
]}

Lu, Xiaoyan ^{[1
]}

Wang, Jiamin ^{[4
]}

Fan, Guanwen ^{[1
]}

Han, Ruiqi ^{[1
]}

Zhang, Bo ^{[1
]}

Zhao, Weili ^{[1
,2
]}

Zhang, Jian ^{[1
]}

机构：

[1] Henan Univ, Sch Mat Sci, Key Lab Special Funct Mat, Minist Educ China, Kaifeng 475004, Peoples R China

[2] Fudan Univ, Inst Integrat Med, Sch Pharm, Shanghai 201203, Peoples R China

[3] Xinxiang Med Univ, Sch Life Sci & Technol, Xinxiang 453003, Peoples R China

[4] Henan Univ, Key Lab Nat Med & Immunoengn Henan Prov, Kaifeng 475004, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2023年 / 96卷 / 01期

基金：

中国国家自然科学基金;

关键词：

CISPLATIN NEPHROTOXICITY; FLUORESCENCE; PHOTOSTABILITY; OUTCOMES; LIVER;

D O I：

10.1021/acs.analchem.3c03967

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fluorescence analysis is an increasingly important contributor to the early diagnosis of kidney diseases. To achieve precise visualization of the kidneys and early diagnosis of related diseases, an asymmetric pyrrolopyrrolidone (DPP) dye platform with C-aromatic substituents and N-lipophilic/hydrophilic modification was constructed. Based on these, we developed the renal-clearable, water-soluble, and kidney injury biomarker leucine aminopeptidase (LAP) activated ratiometric fluorescent probe DPP-S-L. In the mouse model of cisplatin-induced acute kidney injury and during the development of type 2 diabetes to diabetic kidney disease, we visualized for the first time the upregulation of LAP in the kidney and urine by dual-channel ratiometric fluorescence signal and diagnosed the kidney injury earlier and more sensitively than blood/urine enzyme detection and tissue analysis. This study showcases an excellent asymmetric DPP dye platform and renal-clearable ratiometric fluorescent probe design strategy that is extended to determination and visualization of other biomarkers for early disease diagnosis.

引用

页码：272 / 280

页数：9