Pulp waste extracted reinforced powder incorporated biodegradable chitosan composite film for enhancing red grape shelf-life

Chitosan (CS) is widely used as a natural biopolymer due to its semi-crystalline structure, good film-forming properties, and easy availability. CS-based composite films are widely used in industry, particularly in the food sector as active food packaging. Despite all of these advantages, their wide range of applications are constrained by poor mechanical properties. Therefore, this work introduced refined bamboo cellulose powder (RBCP), a reinforcing material that is extracted from waste bamboo pulp and applied to CS composite films to enhance their mechanical and physicochemical properties. The chemical composition and crystallinity properties of CS composite films with RBCP addition were observed by ATR-FTIR and XRD. The homogeneous and heterogeneous surfaces of the RBCP incorporated films before biodegradation and after biodegradation (20 days) were observed by scanning electron microscopy (SEM). The increase in reinforcing RBCP materials from 0.00 to 5.00 % resulted in an increase in tensile strength for CS/RBCP films from 2.9 to 8.3 MPa. The application of the CS/RBCP/5 composite film as red grapefruit storage was also investigated, which performed much better than commercial plastic and control CS films with 92.8 and 88.6 % viability of S. aureus and E. coli bacteria. Overall achieved properties demonstrated strong potential for usage as active packaging materials to preserve and lengthen the shelf life of red grapefruits.