Multilocus sequence typing of L. bulgaricus and S. thermophilus strains from Turkish traditional yoghurts and characterisation of their techno-functional roles

被引：2

作者：

Dikmen, Hilal ^{[1
]}

Goktas, Hamza ^{[2
]}

Demirbas, Fatmanur ^{[1
]}

Kayacan, Selma ^{[1
]}

Ispirli, Humeyra ^{[3
]}

Arici, Muhammet ^{[1
]}

Turker, Mustafa ^{[4
]}

Sagdic, Osman ^{[1
]}

Dertli, Enes ^{[1
]}

机构：

[1] Yildiz Tech Univ, Chem & Met Fac, Food Engn Dept, Istanbul, Turkiye

[2] Istinye Univ, Vocat Sch, Food Technol Programme, Topkapi Campus, Istanbul, Turkiye

[3] Bayburt Univ, Cent Res Lab, Bayburt, Turkiye

[4] Pak Food Prod Mkt, Kocaeli, Turkiye

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2024年 / 33卷 / 03期

关键词：

Turkish yoghurt; MLST; S; thermophilus and L; bulgaricus EPS; Probiotic properties; DELBRUECKII SSP BULGARICUS; LACTIC-ACID BACTERIA; STREPTOCOCCUS-THERMOPHILUS; LACTOBACILLUS-BULGARICUS; IN-VITRO; YEASTS; FOODS; IDENTIFICATION; POPULATION; CHINA;

D O I：

10.1007/s10068-023-01366-2

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this study, Streptococcus thermophilus and Lactobacillus bulgaricus strains from traditional Turkish yoghurts were isolated, identified by 16S rRNA sequencing and genotypically 14 S. thermophilus and 6 L. bulgaricus strains were obtained as distinct strains by MLST analysis. Lactic acid production levels of the L. bulgaricus strains were higher than S. thermophilus strains. HPLC analysis showed that EPS monosaccharide composition of the strains mainly consisted of glucose and galactose. In general, all strains were found to be susceptible for antibiotics, except some strains were resistance to gentamicin and kanamycin. Apart from two strains of S. thermophilus, all strains displayed strong auto-aggregation level greater than 95% at 24 h incubation. S. thermophilus strains showed higher cell surface hydrophobicity than L. bulgaricus strains. This study demonstrated the isolation, identification, genotypic discrimination and techno-functional features of wild type yoghurt starter cultures which can potentially find place in industrial applications.

引用

页码：625 / 635

页数：11

共 7 条

[1] Multilocus sequence typing of L. bulgaricus and S. thermophilus strains from Turkish traditional yoghurts and characterisation of their techno-functional roles
Hilal Dikmen
Hamza Goktas
Fatmanur Demirbas
Selma Kayacan
Humeyra Ispirli
Muhammet Arici
Mustafa Turker
Osman Sagdic
Enes Dertli
Food Science and Biotechnology, 2024, 33 : 625 - 635
[2] Comparative typing of L. delbrueckii subsp bulgaricus strains using multilocus sequence typing and RAPD-PCR
Cebeci, Aysun
Gurakan, G. Candan
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2011, 233 (03) : 377 - 385
[3] Comparative typing of L. delbrueckii subsp. bulgaricus strains using multilocus sequence typing and RAPD–PCR
Aysun Cebeci
G. Candan Gürakan
European Food Research and Technology, 2011, 233 : 377 - 385
[4] Genetic diversity and phylogenetic relationships of Streptococcus thermophilus isolates from traditional Turkish yogurt: multilocus sequence typing (MLST)
Deniz Kiraz
Ali Özcan
Artun Yibar
Enes Dertli
Archives of Microbiology, 2024, 206
[5] Genetic diversity and phylogenetic relationships of Streptococcus thermophilus isolates from traditional Turkish yogurt: multilocus sequence typing (MLST)
Kiraz, Deniz
Ozcan, Ali
Yibar, Artun
Dertli, Enes
ARCHIVES OF MICROBIOLOGY, 2024, 206 (03)
[6] Multilocus sequence typing of seven genetic loci to discriminate strains of L. donovani isolated from Bangladesh
Banu, S. S.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2016, 45 : 61 - 61
[7] Determination of Lactic Acid Production and Antagonistic Activity against Helicobacter pylori of L. delbrueckii subsp bulgaricus and S. thermophilus Strains
Aslim, Belma
Onbasili, Dilsad
Yuksekdag, Zehra Nur
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, 2011, 17 (04) : 609 - 614

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