Robust production of active Ulp1 (SUMO protease) from inclusion bodies

被引：1
|
作者：
Patakottu, Bala Kota Reddy [1 ]
Vedire, Vikram Reddy [1 ]
Reddy, Chereddy Ramamohan [1 ]
机构：
[1] LOKA Biosci Pvt Ltd, ALEAP Ind Estate, Rd 11, PLOT 14 & 15, Pragati Nagar, Hyderabad 500090, India
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2023年 / 211卷
关键词：
Ulp1; SUMO (small ubiquitin like modifier protein); E; coli BL21(DE3);
D O I：
10.1016/j.pep.2023.106328
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High yield purification of Ulp1 is required during the isolation and purification of SUMO-tagged recombinant proteins. However, when expressed as a soluble protein, Ulp1 is toxic to E. coli host cells and most of the protein forms inclusion bodies. The extraction of insoluble Ulp1 followed by its purification and refolding into its active form is a lengthy and costly procedure. In our present study, we developed a simple, cost effective procedure for the large scale production of active Ulp1 that can be used for industrial scale requirements.
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页数：4
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