Kitasatospora fiedleri sp. nov., a novel antibiotic-producing member of the genus Kitasatospora

Strain TU4103(T) was originally sampled from Java, Indonesia and deposited in the Tubingen strain collection under the name 'Streptomyces sp.'. The strain was found to be an antibiotic producer as strain TU4103(T) showed bioactivity against Gram-positive bacteria, such as Bacillus subtilis and Kocuria rhizophila in bioassays. Strain TU4103(T) showed 16S rRNA gene sequence similarity of 99.65% to Kitasatospora cheerisanensis DSM 101999(T) and 98.82% to Kitasatospora niigatensis DSM 44781(T) and Kitasatospora cineracea DSM 44780(T). Genome-based phylogenetic analysis revealed that strain TU4103(T) is closely related to K. cineracea DSM 44780(T) and K. niigatensis DSM 44781(T). The digital DNA-DNA hybridization values between the genome sequences of strain TU4103(T) and its closest phylogenomic relatives, strains DSM 44780(T) and DSM 44781(T), were 43.0 and 42.9 %, respectively. Average nucleotide identity (ANI) values support this claim, with the highest ANI score of 91.14% between TU4103(T) and K. niigatensis being closely followed by an ANI value of 91.10% between K. cineracea and TU4103(T). The genome of TU4103(T) has a size of 7.91 Mb with a G+C content of 74.05 mol%. Whole-cell hydrolysates of strain TU4103(T) are rich in meso-diaminopimelic acid, and rhamnose, galactose and mannose are characteristic as whole-cell sugars. The phospholipid profile contains phosphatidylethanolamine, diphosphatidylglycerol and glycophospholipid. The predominant menaquinones (>93.5 %) are MK-9(H-8) and MK-9(H-6). Based on the phenotypic, genotypic and genomic characteristics, strain TU4103(T) (=DSM 114396(T)=CECT 30712(T)) merits recognition as the type strain of a novel species of the genus Kitasatospora, for which the name Kitasatospora fiedleri sp. nov. is proposed.