Preparation and Recognition Features of Molecularly Imprinted Polymer Membrane for Lamotrigine in Plasma

The molecularly imprinted polymers membranes (MIPMs) were prepared for selective adsorption of lamotrigine (LTG) in plasma by surface molecular imprinting technology with polyvinylidenefluoride (PVDF) membranes as supporter, lamotrigine as template molecule, methyl methacrylate as functional monomer, ethylene glycol dimethacrylate as cross-linking agent, azodiisobutyronitrile as initiator and acetonitrile-dimethylformamide (1:1.5, V/V) as pore-forming agent. The prepared MIPMs were characterized by scanning electron microscope, Fourier transform infrared spectroscopy, Brunaner-emmet-teller measurements, X-ray photoelectron spectroscopy, and thermogravimetric analysis. The adsorption properties of the materials were investigated by kinetic adsorption, isothermal adsorption, selective adsorption, adsorption-desorption and reusability experiments. The results showed that the imprinted layer of LTG was successfully coated on the surface of PVDF, and the materials had uniform particle size. The adsorption capacity and imprinting factor of the MIPMs towards LTG were 3.77 mg/g and 8.97, respectively. The nanomaterials showed fast mass transfer rate (30 min) and good reusability (the adsorption efficiency was 86.66% after 6 cycles), and could be used for the adsorption of LTG in plasma with low matrix interference, recoveries of 86.54%-90.48% and RSD of 1.51%-3.15% (n=5). The proposed LTG MIPMs were demonstrated to be simple and environment friendly, and had high selectivity in rapid separation and extraction of LTG in plasma.