One-day thermal regime extends the lifespan in Caenorhabditis elegans

Environmental factors, including temperature, can modulate an animal's lifespan. However, their under-lying mechanisms remain largely undefined. We observed a profound effect of temperature on the aging of Caenorhabditis elegans (C. elegans) by performing proteomic analysis at different time points (young adult, middle age, and old age) and temperature conditions (20 degrees C and 25 degrees C). Importantly, although at the higher temperature, animals had short life spans, the shift from 20 degrees C to 25 degrees C for one day during early adulthood was beneficial for protein homeostasis since; it decreased protein synthesis and increased degradation. Consistent with our findings, animals who lived longer in the 25 degrees C shift were also more resistant to high temperatures along with oxidative and UV stresses. Furthermore, the lifespan extension by the 25 degrees C shift was mediated by three important transcription factors, namely FOXO/DAF-16, HSF-1, and HIF-1. We re-vealed an unexpected and complicated mechanism underlying the effects of temperature on aging, which could potentially aid in developing strategies to treat age-related diseases. Our data are available in ProteomeXchange with the identifier PXD024916.(c) 2022 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creative-commons.org/licenses/by-nc-nd/4.0/).