Reshaping the Substrate Binding Pocket of β-Amino Acid Dehydrogenase for the Synthesis of Aromatic β-Amino Acids

被引：0

作者：

Liu, Na ^{[1
]}

Feng, Jinhui ^{[1
]}

Chen, Xi ^{[1
]}

Luo, Yuyang ^{[1
,2
]}

Lv, Tong ^{[1
]}

Wu, Qiaqing ^{[1
]}

Zhu, Dunming ^{[1
]}

机构：

[1] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Res Ctr Ind Enzymes, Tianjin Engn Res Ctr Biocatalyt Technol,Key Lab En, Tianjin 300308, Peoples R China

[2] Tianjin Univ Sci & Technol, Sch Biotechnol, State Key Lab Food Nutr & Safety, Key Lab Ind Fermentat Microbiol,Minist Educ, Tianjin 300457, Peoples R China

来源：

ORGANIC LETTERS | 2023年 / 25卷 / 47期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

CATALYTIC ASYMMETRIC-SYNTHESIS; PEPTIDE;

D O I：

10.1021/acs.orglett.3c03366

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

By reshaping the substrate-binding pocket of beta-amino acid dehydrogenase (beta-AADH), some variants were obtained with up to 2560-fold enhanced activity toward the model substrates (S)-beta-homophenylalanine and (R)-beta-phenylalanine. A few aromatic beta-amino acids were prepared with >99% ee and high isolated yields via either kinetic resolution of racemates or reductive amination of the corresponding beta-keto acids. This work expands the catalytic capability of beta-AADHs and highlights their practical application in the synthesis of pharmaceutically relevant beta-amino acids.

引用

页码：8469 / 8473

页数：5

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