Enzyme Cocktails Catalyzed Bioconversion of Lignocellulosic Biomass into Bioethanol

Sustainable production of biofuels from lignocellulosic biomass (LBM) is considered one of the promising solutions to solve the current energy crisis caused by over-reliance on fossil fuels. Enzymatic hydrolysis is a crucial step in determining the efficient conversion of biomass cellulose into biofuels as well as high value-added chemicals, and it has the characteristics of efficient reaction, economy, and environmental friendliness. With the advancement of enzyme engineering technology, enzymes have been endowed with more functions, such as mixed substrate utilization, inhibitor resistance and specific metabolism, called enzyme cocktails. This Paper introduces the typical procedure of enzymatic hydrolysis of LBM and discusses their applications in hybrid LBM hydrolysis. In addition, the challenges of enzymatic LBM hydrolysis and possible development directions are outlined to guide integrated enzymatic strategies in biorefinery processes.