Novel ethyl p-methoxy cinnamate rich Kaempferia galanga (L.) essential oil and its pharmacological applications: special emphasis on anticholinesterase, anti-tyrosinase, a-amylase inhibitory, and genotoxic efficiencies

Background: Kaempferia galanga (L.) is one of the prospective therapeutic plants with an aromatic rhizome, and belongs to the Zingiberaceae family. This herb is commonly used by local practitioners in traditional Asian medicine.Methods: In the present investigation, the novel Kaempferia galanga rhizome essential oil rich in ethyl p-methoxy cinnamate (EMCKG) was evaluated using GC/MS for chemical composition analysis. EMCKG was analyzed for its possible antimicrobial, neurodegenerative inhibitory, acetylcholinesterase, anti-inflammatory, and antioxidant activities as well as for the genotoxic effects using the standard methodologies. ANOVA and post hoc was performed to test the statistical significance of the study.Results: GC/MS analysis identified ethyl p-methoxy cinnamate as the major component of EMCKG essential oil with an area percentage of 66.39%. The EMCKG exhibited moderate (DPPH assay IC50 = 15.64 +/- 0.263 mu g/mL; ABTS assay IC50 = 16.93 +/- 0.228 mu g/mL) antioxidant activity than standard ascorbic acid (DPPH assay IC50 = 21.24 +/- 0.413 mu g/mL; ABTS assay IC50 = 21.156 +/- 0.345 mu g/mL). Similarly, EMCKG showed comparable activity in albumin denaturation (IC50 = 2.93 +/- 0.59 mu g/mL) and protease inhibitor assay (IC50 = 17.143 +/- 0.506 mu g/mL) to that of standard sodium diclofenac (IC50 = 23.87 +/- 0.729 mu g/mL and IC50 = 19.18 +/- 0.271 mu g/mL, respectively). The EMCKG exhibited a dose-dependent antimicrobial activity pattern with the highest inhibitory activity at 500 mu g/mL against Staphylococcus aureus and considerable anticholinesterase activities (IC50 = 21.94 +/- 0.109 mu g/mL) compared to the standard galanthamine (IC50 = 27.18 +/- 0.511 mu g/mL). EMCKG also showed strong anti-diabetic activity (IC50 = 18.503 +/- 0.480 mu g/mL) and anti-tyrosinase activity (IC50 = 14.756 +/- 0.325 mu g/mL) as compared to the standards used (acarbose IC50 = 20.39 +/- 0.231 mu g/mL and kojic acid IC50 = 17.73 +/- 0.192 mu g/ mL) in the study. Genotoxicity analysis of EMCKG revealed that at 1 mu g/mL concentration has no toxic effects in mitosis of Allium cepa roots (Mitotic Index MI = 13.56% and chromosomal aberration CA = 07.60%). The ANOVA confirmedthat except for the anticholinesterase activity, there is insignificant difference for essential oil and standards used for all the other bioactivities thus confirming their interchangeable applicability. Conclusions: Current research provides the basis for the fact that besides being a rich source of ethyl p-methoxycinnamate, EMCKG has the potential for future formulation and development of an inexpensive skin-care agent and for the preparation of anti-diabetic drugs.