The Effect of Polyene Antibiotic Amphotericin B on Erythrocyte Cytoarchitectonics and Osmotic Resistance

被引：1
|
作者：
Sokolova, L. O. [1 ]
Kalaeva, E. A. [1 ]
Artyukhov, V. G. [1 ]
Putintseva, O. V. [1 ]
Nakvasina, M. A. [1 ]
Litvinov, N. V. [1 ]
Gizetdinova, L. R. [1 ]
机构：
[1] Voronezh State Univ, Voronezh, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2023年 / 175卷 / 4期
关键词：
red blood cells; osmotic resistance of erythrocytes; amphotericin B; kinetics of hypoosmotic hemolysis; scanning electron microscopy;
D O I：
10.1007/s10517-023-05888-x
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We studied the effect of amphotericin B (2.5x10-5 and 5.4x10-5 M) on osmotic resistance and surface cytoarchitectonics of donor blood erythrocytes. Antibiotic at a concentration of 2.5x10-5 M induced most pronounced changes in the studied parameters, which can be related to the specifics of the spatial organization of the cholesterol-amphotericin B complexes at different stoichiometric ratios of the components and their ability to pore formation in the membranes. Cholesterol binding to the polyene antibiotic and the appearance of perforations in the plasma membrane lead to accumulation of reversibly and irreversibly deformed cells and their hemolysis. The appearance of a large number of irreversibly deformed erythrocytes indicates an impaired ability to elastic deformation in the microcirculatory stream, which can lead to disruption of their functions in vivo and intravascular hemolysis.
引用
收藏
页码：468 / 472
页数：5
相关论文
共 50 条
  • [1] The Effect of Polyene Antibiotic Amphotericin B on Erythrocyte Cytoarchitectonics and Osmotic Resistance
    L. O. Sokolova
    E. A. Kalaeva
    V. G. Artyukhov
    O. V. Putintseva
    M. A. Nakvasina
    N. V. Litvinov
    L. R. Gizetdinova
    Bulletin of Experimental Biology and Medicine, 2023, 175 : 468 - 472
  • [2] Dimers of polyene antibiotic amphotericin B
    Gruszecki, WI
    Herec, M
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2003, 72 (1-3) : 103 - 105
  • [3] Oxidized forms of polyene antibiotic amphotericin B
    Gagos, Mariusz
    Czernel, Grzegorz
    CHEMICAL PHYSICS LETTERS, 2014, 598 : 5 - 9
  • [4] EFFECT OF AGGREGATION ON THE KINETICS OF AUTOXIDATION OF THE POLYENE ANTIBIOTIC AMPHOTERICIN-B
    LAMYFREUND, MT
    FERREIRA, VFN
    FALJONIALARIO, A
    SCHREIER, S
    JOURNAL OF PHARMACEUTICAL SCIENCES, 1993, 82 (02) : 162 - 166
  • [5] The Contribution of Raman Scattering to the Fluorescence of the Polyene Antibiotic Amphotericin B
    Bolard, Jacques
    Cheron, Monique
    Cleary, John D.
    Kramer, Robert E.
    JOURNAL OF FLUORESCENCE, 2011, 21 (02) : 831 - 834
  • [6] Evidence that impurities contribute to the fluorescence of the polyene antibiotic amphotericin B
    Bolard, Jacques
    Cleary, John D.
    Kramer, Robert E.
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2009, 63 (05) : 921 - 927
  • [7] The Contribution of Raman Scattering to the Fluorescence of the Polyene Antibiotic Amphotericin B
    Jacques Bolard
    Monique Chéron
    John D. Cleary
    Robert E. Kramer
    Journal of Fluorescence, 2011, 21 : 831 - 834
  • [8] Reconstituted high density lipoprotein enriched with the polyene antibiotic amphotericin B
    Oda, MN
    Hargreaves, PL
    Beckstead, JA
    Redmond, KA
    van Antwerpen, R
    Ryan, RO
    JOURNAL OF LIPID RESEARCH, 2006, 47 (02) : 260 - 267
  • [9] STRUCTURE AND ABSOLUTE CONFIGURATION OF POLYENE MACROLIDE ANTIBIOTIC AMPHOTERICIN-B
    MECHLINSKI, W
    SCHAFFNER, CP
    GANIS, P
    AVITABILE, G
    TETRAHEDRON LETTERS, 1970, (44) : 3873 - +
  • [10] Organization of polyene antibiotic amphotericin B at the argon-water interface
    Gagos, Mariusz
    Gruszecki, Wieslaw I.
    BIOPHYSICAL CHEMISTRY, 2008, 137 (2-3) : 110 - 115
12345