. Renal failure disease is defined as a reduced glomerular filtration rate and increased urine albumin excretion, as well as peripheral edema, anemia, bone diseases, and metabolic hazards. Hemodialysis means removing the toxins and waste from the blood due to the inability of the kidney to do this. The aim was measuring the level of some biochemical markers in Iraqi renal failure patients on hemodialysis and comparing them with healthy people. This study included 63 patients with renal failure on hemodialysis in addition to 40 healthy individuals as a control group. Fasting blood sugar, hemoglobin, urea, creatinine, protein, albumin, potassium, chloride, cholesterol, triglyceride, alanine aminotransferase ALT/GPT, and aspartate aminotransferase AST/GOT were measured. The results showed a significant increase in fasting blood sugar level, urea, creatinine, potassium, chloride, choles-terol, triglyceride, bilirubin and GOT. While the results showed a decrease in the level of hemoglobin, protein, and GPT.