Novel CRISPR/Cas9 system assisted by fluorescence marker and pollen killer for high-efficiency isolation of transgene-free edited plants in rice

被引：1

作者：

Yu, Dong ^{[1
]}

Zhou, Tianshun ^{[1
,2
]}

Xu, Na ^{[1
]}

Sun, Xuewu ^{[1
]}

Song, Shufeng ^{[1
]}

Liu, Hai ^{[1
]}

Sun, Zhizhong ^{[1
]}

Lv, Qiming ^{[1
]}

Chen, Jin ^{[1
]}

Tan, Yanning ^{[1
]}

Sheng, Xiabing ^{[1
]}

Li, Li ^{[1
,2
]}

Yuan, Dingyang ^{[1
,2
]}

机构：

[1] Hunan Acad Agr Sci, Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Peoples R China

[2] Hunan Univ, Coll Biol, Longping Branch, Changsha, Peoples R China

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2024年 / 22卷 / 06期

关键词：

CRISPR/; Cas9; Transgene-free; Fluorescence Marker; Pollen Killer; Rice;

D O I：

10.1111/pbi.14293

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

[No abstract available]

引用

页码：1649 / 1651

页数：3

共 28 条

[1] Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants
He, Yubing
Zhu, Min
Wang, Lihao
Wu, Junhua
Wang, Qiaoyan
Wang, Rongchen
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[2] Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants
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ZHU Min
WANG Lihao
WU Junhua
WANG Qiaoyan
WANG Rongchen
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Rice Science, 2019, (02) : 109 - 117
[3] Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants
He Yubing
Zhu Min
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Wu Junhua
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RICE SCIENCE, 2019, 26 (02) : 109 - 117
[4] Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants
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Rice Science, 2019, 26 (02) : 109 - 117
[5] Generation of Transgene-Free Maize Male Sterile Lines Using the CRISPR/Cas9 System
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Wang, Guoying
Liu, Yunjun
FRONTIERS IN PLANT SCIENCE, 2018, 9
[6] Generation of transgene-free genome-edited carrot plants using CRISPR/Cas9-RNP complexes
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[7] H2O2-Based Method for Rapid Detection of Transgene-Free Rice Plants from Segregating CRISPR/Cas9 Genome-Edited Progenies
Wu, Tsung-Meng
Huang, Jian-Zhi
Oung, Hui-Min
Hsu, Yi-Ting
Tsai, Yu-Chang
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (16)
[8] Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds
Aliaga-Franco, Norma
Zhang, Cunjin
Presa, Silvia
Srivastava, Anjil K.
Granell, Antonio
Alabadi, David
Sadanandom, Ari
Blazquez, Miguel A.
Minguet, Eugenio G.
FRONTIERS IN PLANT SCIENCE, 2019, 10
[9] Efficient and transgene-free gene targeting using Agrobacterium-mediated delivery of the CRISPR/Cas9 system in tomato
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Perrot, Laura
Mara, Kostlend
Botton, Emmanuel
Nogue, Fabien
Mazier, Marianne
PLANT CELL REPORTS, 2019, 38 (04) : 459 - 462
[10] Efficient and transgene-free gene targeting using Agrobacterium-mediated delivery of the CRISPR/Cas9 system in tomato
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Plant Cell Reports, 2019, 38 : 459 - 462

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