LncRNA-adm2 targets adm2 via cid-miR-n3 and negatively regulates the inflammatory response in grass carp (Ctenopharyngodon idella)

被引：2
|
作者：
Niu, Huiqin [1 ,2 ,3 ]
Hao, Yinghu [1 ,2 ,3 ]
Pang, Yifan [1 ,2 ,3 ]
Shen, Yubang [1 ,2 ,3 ]
Li, Jiale [1 ,2 ,3 ,4 ]
Xu, Xiaoyan [1 ,2 ,3 ,4 ]
机构：
[1] Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr & Rural Affairs, Shanghai, Peoples R China
[2] Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai, Peoples R China
[3] Shanghai Ocean Univ, Shanghai Engn Res Ctr Aquaculture, Shanghai, Peoples R China
[4] Shanghai Ocean Univ, Coll Aquaculture & Life Sci, Shanghai 201306, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2023年 / 138卷
基金：
中国国家自然科学基金;
关键词：
Ctenopharyngodon idella; lncRNA-adm2; adm2; Aeromonas hydrophila; Inflammation; LONG NONCODING RNAS; EXPRESSION; MICRORNAS; INFECTION; IMMUNITY;
D O I：
10.1016/j.fsi.2023.108800
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Long non-coding RNAs (lncRNAs), which impact gene expression following pathogen infections, have garnered significant attention in recent years. Recent discoveries have revealed that lncRNAs play a crucial role in fish immune responses to pathogen infections. We investigated the influence of lncRNA-adm2 on the antibacterial immune response generated by Aeromonas hydrophila in grass carp (Ctenopharyngodon idella) through the adsorption of cid-miR-n3. Furthermore, we found that cid-miR-n3 interacts with lncRNA-adm2 and targets the 3' UTR of adm2. The upregulation of lncRNA-adm2 expression led to the suppression of pro-inflammatory cytokines (il-1 beta and il-6) in CIK cells, while anti-inflammatory cytokines (il-10) increased. Our research provides evidence that lncRNAs are involved in the antibacterial immune response of fish, expanding our understanding of the function of lncRNAs in teleosts.
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页数：8
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