Imaging cellular forces with photonic crystals

被引：15

作者：

Li, Qiwei ^{[1
]}

Chen, Zaozao ^{[1
,2
]}

Zhang, Ying ^{[1
]}

Ding, Shuang ^{[1
]}

Ding, Haibo ^{[1
]}

Wang, Luping ^{[1
,3
]}

Xie, Zhuoying ^{[1
]}

Fu, Yifu ^{[1
]}

Wei, Mengxiao ^{[1
]}

Liu, Shengnan ^{[1
]}

Chen, Jialun ^{[1
]}

Wang, Xuan ^{[1
]}

Gu, Zhongze ^{[1
,2
]}

机构：

[1] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Digital Med Engn, Nanjing 210096, Jiangsu, Peoples R China

[2] Southeast Univ, Inst Biomat & Med Devices, Suzhou 215163, Jiangsu, Peoples R China

[3] Ningbo Univ, Fac Sports Sci, Ningbo 315211, Peoples R China

来源：

NATURE COMMUNICATIONS | 2023年 / 14卷 / 01期

关键词：

TRACTION FORCES; CELLS; CYTOSKELETON; MUSCLE; GUIDE;

D O I：

10.1038/s41467-023-43090-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Current techniques for visualizing and quantifying cellular forces have limitations in live cell imaging, throughput, and multi-scale analysis, which impede progress in cell force research and its practical applications. We developed a photonic crystal cellular force microscopy (PCCFM) to image vertical cell forces over a wide field of view (1.3mm x 1.0mm, a 10 x objective image) at high speed (about 20 frames per second) without references. The photonic crystal hydrogel substrate (PCS) converts micro-nano deformations into perceivable color changes, enabling in situ visualization and quantification of tiny vertical cell forces with high throughput. It enabled long-term, cross-scale monitoring from subcellular focal adhesions to tissue-level cell sheets and aggregates.

引用

页数：14

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