Incidental Detection of a Chromosomal Aberration by Array-CGH in an Early Prenatal Diagnosis for Monogenic Disease on Coelomic Fluid

被引:1
|
作者
Vinciguerra, Margherita [1 ]
Leto, Filippo [1 ]
Cassara, Filippo [1 ]
Tartaglia, Viviana [1 ]
Malacarne, Michela [2 ]
Coviello, Domenico [2 ]
Cigna, Valentina [3 ]
Orlandi, Emanuela [3 ]
Picciotto, Francesco [3 ]
Cucinella, Gaspare [3 ]
Salzano, Emanuela [4 ]
Piccione, Maria [4 ]
Maggio, Aurelio [5 ]
Giambona, Antonino [1 ]
机构
[1] Azienda Ospedaliera Ospedali Riuniti Villa Sofia C, Unit Mol Diag Rare Hematol Dis, I-90146 Palermo, Italy
[2] Ist Giannina Gaslini, Lab Human Genet, I-16147 Genoa, Italy
[3] Azienda Ospedaliera Ospedali Riuniti Villa Sofia C, Unit Fetal Med & Prenatal Diag, I-90146 Palermo, Italy
[4] Azienda Ospedaliera Ospedali Riuniti Villa Sofia C, Med Genet Unit, I-90146 Palermo, Italy
[5] Azienda Ospedaliera Ospedali Riuniti Villa Sofia C, Unit Hematol Rare Dis Blood & Blood Forming Organs, I-90146 Palermo, Italy
来源
LIFE-BASEL | 2023年 / 13卷 / 01期
关键词
prenatal diagnosis; array comparative genomic hybridization; coelocentesis; monosomy X; beta thalassemia; COELOCENTESIS; GENE;
D O I
10.3390/life13010020
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Turner syndrome is a rare genetic condition in which a female is partly or completely missing an X chromosome. Signs and symptoms vary among those affected. In fetuses that survive at birth and without congenital malformations, the prognosis is usually positive, but it has high lethality in utero, especially in the first trimester of pregnancy. Methods: We report a case of monosomy X detected during a prenatal diagnosis for beta thalassemia on coelomic fluid (CF) at the VIII week of gestation. Beta globin gene analysis, whole genome amplification (WGA), quantitative fluorescent PCR and array comparative genomic hybridization (array-CGH) were performed on DNA extracted from CF. Results: A monoallelic pattern of all Short Tandem Repeats mapped on the X chromosome was found and array-CGH performed on WGA from a few fetal erythroblasts confirmed monosomy X. Conclusion: This report underlines the importance of an early prenatal diagnosis and the countless potentialities of array-CGH that could make definition of molecular karyotype possible from a few fetal cells, unlike conventional cytogenetic techniques that require a greater cellular content. This is the first report of a molecular karyotype obtained from two cells selected by micromanipulation of CF and defined at such an early gestational age.
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页数:11
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