A phosphamide nucleotide analog: a substrate for polymerase synthesis of DNA

被引：0

作者：

Meng, Jiong ^{[1
]}

Guo, Qiaqia ^{[1
]}

Zhai, Xiaona ^{[1
]}

Yang, Song ^{[1
]}

Wang, Shuai ^{[1
]}

Wang, Pengcheng ^{[1
]}

Ji, Debin ^{[1
]}

机构：

[1] Shandong Univ, Inst Frontier Chem, Sch Chem & Chem Engn, Qingdao 266237, Peoples R China

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2024年 / 22卷 / 15期

基金：

中国国家自然科学基金;

关键词：

HYDROLYSIS;

D O I：

10.1039/d4ob00089g

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A type of modified nucleotide, deoxynucleotide gamma-amidotriphosphates (dNTP gamma NH2s), exhibited around five times higher stability than dNTPs. These phosphamide nucleotides can be utilized by several DNA polymerases, and the amplification of a 10 kb DNA fragment through the polymerase chain reaction (PCR) can be accomplished even under conditions of high temperature, extended storage, or repeated freeze-thaw cycles. However, the control PCR with standard dNTPs was unsuccessful. These results indicate that dNTP gamma NH2s have the potential to substitute dNTPs in PCR. A phosphamide nucleotide analog (dNTP gamma NH2) exhibited higher stability than dNTPs and can be recognized by DNA polymerases in PCR.

引用

页码：2963 / 2967

页数：5

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