Hydrogel Bioinks of Alginate and Curcumin-Loaded Cellulose Ester-Based Particles for the Biofabrication of Drug-Releasing Living Tissue Analogs

被引：13

作者：

Carvalho, Joao P. F. ^{[1
]}

Teixeira, Maria C. ^{[1
]}

Lameirinhas, Nicole S. ^{[1
]}

Matos, Filipe S. ^{[1
]}

Luis, Jorge L. ^{[2
]}

Pires, Liliana ^{[2
]}

Oliveira, Helena ^{[3
,4
]}

Oliveira, Martinho ^{[2
]}

Silvestre, Armando J. D. ^{[1
]}

Vilela, Carla ^{[1
]}

Freire, Carmen S. R. ^{[1
]}

机构：

[1] Univ Aveiro, Dept Chem, CICECO Aveiro Inst Mat, P-3810193 Aveiro, Portugal

[2] Univ Aveiro, Sch Design Management & Prod Technol Northern Ave, EMaRT Grp Emerging Mat Res Technol, CICECO Aveiro Inst Mat, P-3720511 Oliveira De Azemeis, Portugal

[3] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal

[4] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal

来源：

ACS APPLIED MATERIALS & INTERFACES | 2023年 / 15卷 / 34期

关键词：

3D bioprinting; composite hydrogel bioinks; cellulose ester-based particles; drug delivery; living tissue analogs; SUSTAINED-RELEASE; BIO-INKS; 3D; NANOPARTICLES; STABILITY; MEMBRANES; SCAFFOLD;

D O I：

10.1021/acsami.3c07077

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

3D bioprinting isa versatile technique that allows the fabricationof living tissue analogs through the layer-by-layer deposition ofcell-laden biomaterials, viz. bioinks. In this work, composite alginatehydrogel-based bioinks reinforced with curcumin-loaded particles ofcellulose esters (CEpCUR) and laden with human keratinocytes (HaCaT)are developed. The addition of the CEpCUR particles, with sizes of740 & PLUSMN; 147 nm, improves the rheological properties of the inks,increasing their shear stress and viscosity, while preserving therecovery rate and the mechanical and viscoelastic properties of theresulting fully cross-linked hydrogels. Moreover, the presence ofthese particles reduces the degradation rate of the hydrogels from26.3 & PLUSMN; 0.8% (ALG) to 18.7 & PLUSMN; 1.3% (ALG:CEpCUR_10%) after3 days in the culture medium. The 3D structures printed with the ALG:CEpCURinks reveal increased printing definition and the ability to releasecurcumin (with nearly 70% of cumulative release after 24 h in PBS).After being laden with HaCaT cells (1.2 x 10(6) cellsmL(-1)), the ALG:CEpCUR bioinks can be successfully3D bioprinted, and the obtained living constructs show good dimensionalstability and high cell viabilities at 7 days post-bioprinting (nearly90%), confirming their great potential for application in fields likewound healing.

引用

页码：40898 / 40912

页数：15