E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT

被引：1
|
作者：
Astanina, Elena [1 ,2 ]
Petrillo, Sara [3 ]
Genova, Tullio [4 ]
Mussano, Federico [5 ]
Bussolino, Federico [1 ,2 ]
机构：
[1] Univ Torino, Dept Oncol, Turin, Italy
[2] IRCCS, Candiolo Canc Inst, FPO, Candiolo, Italy
[3] Univ Torino, Dept Mol Biotechnol & Hlth Sci, Mol Biotechnol Ctr, Turin, Italy
[4] Univ Torino, Dept Life Sci & Syst Biol, Turin, Italy
[5] Univ Torino, CIR Dent Sch, Dept Surg Sci, Via Nizza 230, I-10126 Turin, Italy
来源：
BIO-PROTOCOL | 2023年 / 13卷 / 09期
关键词：
Imaging; X-ray computed tomography; Micro-CT; 3D visualization; Mouse embryo; Heart development;
D O I：
10.21769/BioProtoc.4662
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
X-ray computed microtomography (mu CT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology and a precise morphometric analysis. Here, we describe a method for 3D visualization and morphometric analysis by mu CT scanning of the embryonic heart of iodine-stained E15.5 mouse embryos.
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页数：6
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