Estimation of Relative Chlorophyll Content in Spring Wheat Based on Multi-Temporal UAV Remote Sensing

Relative chlorophyll content (SPAD) is an important index for characterizing the nitrogen nutrient status of plants. Continuous, rapid, nondestructive, and accurate estimation of SPAD values in wheat after heading stage can positively impact subsequent nitrogen fertilization management strategies, which regulate grain filling and yield quality formation. In this study, the estimation of SPAD of leaf relative chlorophyll content in spring wheat was conducted at the experimental base in Wuyuan County, Inner Mongolia in 2021. Multispectral images of different nitrogen application levels at 7, 14, 21, and 28 days after the wheat heading stage were acquired by DJI P4M UAV. A total of 26 multispectral vegetation indices were constructed, and the measured SPAD values of wheat on the ground were obtained simultaneously using a handheld chlorophyll meter. Four machine learning algorithms, including deep neural networks (DNN), partial least squares (PLS), random forest (RF), and Adaptive Boosting (Ada) were used to construct SPAD value estimation models at different time from heading growth stages. The model's progress was evaluated by the coefficient of determination (R-2), root mean square error (RMSE), and mean absolute error (MAPE). The results showed that the optimal SPAD value estimation models for different periods of independent reproductive growth stages of wheat were different, with PLS as the optimal estimation model at 7 and 14 days after heading, RF as the optimal estimation model at 21 days after heading, and Ada as the optimal estimation model at 28 d after heading. The highest accuracy was achieved using the PLS model for estimating SPAD values at 14 d after heading (training set R-2 = 0.767, RMSE = 3.205, MAPE = 0.060, and R-2 = 0.878, RMSE = 2.405, MAPE = 0.045 for the test set). The combined analysis concluded that selecting multiple vegetation indices as input variables of the model at 14 d after heading stage and using the PLS model can significantly improve the accuracy of SPAD value estimation, provides a new technical support for rapid and accurate monitoring of SPAD values in spring wheat.