Study of anti-inflammatory and anti-arthritic potential of curcumin-loaded Eudragit L100 and hydroxy propyl methyl cellulose (HPMC) microparticles

Curcumin is derived from Curcuma longa's dried root rhizomes possessing extensive variety of therapeutic actions such as antifungal, anti-inflammatory, antibacterial, antispasmodic, antioxidant and anti-arthritic. The current study was conducted to fabricate, characterize and optimize curcumin-loaded Eudragit L-100 and hydroxy propyl methyl cellulose (HPMC) sustained release and improved bioavailable microparticles for anti-arthritis and anti-inflammatory activities. Curcumin-loaded microparticles made with Eudragit L100 and HPMC via ESE having different polymer and emulsifier concentrations. Optical microscope showed spherical microparticles, and FTIR spectroscopic analysis displayed characteristic peaks at 3519 cm(-1), 1366 cm(-1), 950 cm(-1) and 728 cm(-1) due to O-H group, C-OH bending and bending vibration of -CH groups, respectively. SEM analysis showed that mean microparticles diameter was 30.2-76.7 mu m having encapsulation efficiency 78.8-96.2%. Curcumin (600 mu g/ml) showed 52%HRBC membrane stabilization and 71% protein denaturation inhibition. Curcumin (600 mu g/ml) produced microparticles with 30.2 mu m-diameter showed highest encapsulation, sustained drug release, significant anti-arthritic potential with good anti-inflammatory activity make it suitable as new therapeutic anti-arthritic agent. From results, it was suggested that this is a promising strategy for the development and treatment of chronic ailments like arthritis and inflammation. [GRAPHICS] .