Biofilm Formation by Nontypeable Haemophilus Influenzae and Resistance to Complement-Mediated Clearance

Biofilm formation has been suggested to be associated with phenotype changes compared with the planktonic form. We screened 1092 Haemophilus influenzae isolates for their genetic relationships and then selected 29 isolates from different genotypes and phenotypes and tested their ability to form biofilm. Our data showed a higher capacity of nontypable isolates, particularly isolates from respiratory and genital infections to form biofilm, compared with typable isolates. This ability to form biofilm was also correlated with reduced deposition of the complement component C3b on biofilm-involved bacteria. These data suggest that the biofilm formation contributes to the virulence of nontypable H. influenzae. Biofilm formation contributes to the pathogenesis of Haemophilius influenzae, particularly nontypable H. influenzae isolates that were recovered from mucosal surfaces. Biofilm formation was correlated with reduced of the complement component C3b deposition on bacteria. Biofilm can be a therapeutic target.