The importance of combining serological testing with RT-PCR assays for efficient detection of COVID-19 and higher diagnostic accuracy

被引:5
|
作者
Alamri, Sawsan S. [1 ]
Alsaieedi, Ahdab [1 ,2 ]
Khouqeer, Yousef [3 ]
Afeef, Marwah [4 ]
Alharbi, Samiyah [5 ]
Algaissi, Abdullah [6 ,7 ]
Alghanmi, Maimonah [1 ,2 ]
Altorki, Tarfa [1 ,2 ]
Zawawi, Ayat [1 ,2 ]
Alfaleh, Mohamed A. [1 ,8 ]
Hashem, Anwar M. [1 ,9 ]
Alhabbab, Rowa [1 ,2 ]
机构
[1] King Abdulaziz Univ, King Fahd Med Res Ctr, Vaccines & Immunotherapy Unit, Jeddah, Saudi Arabia
[2] King Abdulaziz Univ, Fac Appl Med Sci, Dept Med Lab Sci, Jeddah, Saudi Arabia
[3] King Saud bin Abdulaziz Univ Hlth Sci, Coll Med, Jeddah, Saudi Arabia
[4] King Fahad Gen Hosp, Study & Res Dept, Jeddah, Saudi Arabia
[5] King Fahad Gen Hosp, Intens Care Unit, Jeddah, Saudi Arabia
[6] Jazan Univ, Coll Appl Med Sci, Dept Med Labs Technol, Jazan, Saudi Arabia
[7] Jazan Univ, Med Res Ctr, Jazan, Saudi Arabia
[8] King Abdulaziz Univ, Fac Pharm, Dept Pharmaceut, Jeddah, Saudi Arabia
[9] King Abdulaziz Univ, Fac Med, Dept Med Microbiol & Parasitol, Jeddah, Saudi Arabia
来源
PEERJ | 2023年 / 11卷
关键词
SARS-CoV-2; Serology; COVID-19; Diagnostic; Testing profile; Infection; RT-PCR; Healthcare worker; SYNDROME CORONAVIRUS 2; VARIANTS; DELTA; BETA;
D O I
10.7717/peerj.15024
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Misdiagnosing suspected COVID-19 individuals could largely contribute to the viruses transmission, therefore, making an accurate diagnosis of infected subjects vital in minimizing and containing the disease. Although RT-PCR is the standard method in detecting COVID-19, it is associated with some limitations, including possible false negative results. Therefore, serological testing has been suggested as a complement assay to RT-PCR to support the diagnosis of acute infections. In this study, 15 out of 639 unvaccinated healthcare workers (HCWs) were tested negative for COVID-19 by RT-PCR and were found seropositive for SARS-CoV-2 nucleocapsid protein-specific IgM and IgG antibodies. These participants underwent additional confirmatory RT-PCR and SARS-CoV-2 spike-specific ELISA tests. Of the 15 individuals, nine participants were found negative by second RT-PCR but seropositive for anti-spike IgM and IgG antibodies and neutralizing antibodies confirming their acute infection. At the time of collection, these nine individuals were in close contact with COVID-19-confirmed patients, with 77.7% reporting COVID-19-related symptoms. These results indicate that including serological tests in the current testing profile can provide better outcomes and help contain the spread of the virus by increasing diagnostic accuracy to prevent future outbreaks rapidly.
引用
收藏
页数:17
相关论文
共 50 条
  • [1] Laboratory Parameters in Detection of COVID-19 Patients with Positive RT-PCR; a Diagnostic Accuracy Study
    Mardani, Rajab
    Vasmehjani, Abbas Ahmadi
    Zali, Fatemeh
    Gholami, Alireza
    Nasab, Seyed Dawood Mousavi
    Kaghazian, Hooman
    Kaviani, Mehdi
    Ahmadi, Nayebali
    [J]. ARCHIVES OF ACADEMIC EMERGENCY MEDICINE, 2020, 8 (01)
  • [2] The Relationship between Serological Testing, Demographics, Clinical Presentation and RT-PCR Testing for COVID-19
    Gelbart, Bradley
    Schapkaitz, Elise
    Kaftel, Steve
    Peretz, Eric
    Peretz, Asi
    [J]. CLINICAL LABORATORY, 2021, 67 (05) : 1293 - 1298
  • [3] Diagnostic accuracy of autoverification and guidance system for COVID-19 RT-PCR results
    Yingmu Cai
    Mengyu Liu
    Zhiyuan Wu
    Cuihong Tian
    Song Qiu
    Zhen Li
    Feng Xu
    Wei Li
    Yan Zheng
    Aijuan Xu
    Longxu Xie
    Xuerui Tan
    [J]. EPMA Journal, 2023, 14 : 119 - 129
  • [4] Diagnostic accuracy of autoverification and guidance system for COVID-19 RT-PCR results
    Cai, Yingmu
    Liu, Mengyu
    Wu, Zhiyuan
    Tian, Cuihong
    Qiu, Song
    Li, Zhen
    Xu, Feng
    Li, Wei
    Zheng, Yan
    Xu, Aijuan
    Xie, Longxu
    Tan, Xuerui
    [J]. EPMA JOURNAL, 2023, 14 (01): : 119 - 129
  • [5] A Compressed Sensing Approach to Pooled RT-PCR Testing for COVID-19 Detection
    Ghosh, Sabyasachi
    Agarwal, Rishi
    Rehan, Mohammad Ali
    Pathak, Shreya
    Agarwal, Pratyush
    Gupta, Yash
    Consul, Sarthak
    Gupta, Nimay
    Ritika
    Goenka, Ritesh
    Rajwade, Ajit
    Gopalkrishnan, Manoj
    [J]. IEEE OPEN JOURNAL OF SIGNAL PROCESSING, 2021, 2 (02): : 248 - 264
  • [6] Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing
    Zowawi, Hosam M.
    Alenazi, Thamer H.
    AlOmaim, Waleed S.
    Wazzan, Ahmad
    Alsufayan, Abdullah
    Hasanain, Rihab A.
    Aldibasi, Omar S.
    Althawadi, Sahar
    Altamimi, Sarah A.
    Mutabagani, Maysoon
    Alamri, Maha
    Almaghrabi, Reem S.
    Al-Abdely, Hail M.
    Memish, Ziad A.
    Alqahtani, Saleh A.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2021, 59 (05)
  • [7] Sensitivity and specificity of 14 SARS-CoV-2 serological assays and their diagnostic potential in RT-PCR negative COVID-19 infections
    Van Honacker, Eveline
    Coorevits, Liselotte
    Boelens, Jerina
    Verhasselt, Bruno
    Van Braeckel, Eva
    Bauters, Fre
    De Bus, Liesbet
    Schelstraete, Petra
    Willems, Jef
    Vandendriessche, Stien
    Padalko, Elizaveta
    [J]. ACTA CLINICA BELGICA, 2022, 77 (02) : 315 - 320
  • [8] A Literature Review on the Relative Diagnostic Accuracy of Chest CT Scans versus RT-PCR Testing for COVID-19 Diagnosis
    Al-Momani, Hafez
    [J]. TOMOGRAPHY, 2024, 10 (06) : 935 - 948
  • [9] A pooled RT-PCR testing strategy for more efficient COVID-19 pandemic management
    Ayaz, Akif
    Demir, Asli Guner Ozturk
    Ozturk, Gurkan
    Kocak, Mehmet
    [J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2022, 116 : 1 - 6
  • [10] Three Novel Real-Time RT-PCR Assays for Detection of COVID-19 Virus
    Niu, Peihua
    Lu, Roujian
    Li Zhao
    Wang, Huijuan
    Huang, Baoying
    Ye, Fei
    Wang, Wenling
    Tan, Wenjie
    [J]. CHINA CDC WEEKLY, 2020, 2 (25): : 453 - 457