Fermentation characteristics and microbial community composition of wet brewer's grains and corn stover mixed silage prepared with cellulase and lactic acid bacteria supplementation

被引:2
|
作者
Zhao, Guoqiang [1 ]
Wu, Hao [2 ]
Li, Yangyuan [1 ]
Li, Li [1 ]
He, Jiajun [1 ]
Yang, Xinjian [1 ]
Xie, Xiangxue [1 ]
机构
[1] Guangdong VTR Biotech Co LTD, Zhuhai 519060, Peoples R China
[2] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China
基金
中国国家自然科学基金;
关键词
Cellulase; Corn Stover; Fermentation Quality; Lactic Acid Bacteria; Microbial Community; Wet Brewer's Grains; AEROBIC STABILITY; CHEMICAL-COMPOSITION; INOCULATION; QUALITY; SILO;
D O I
10.5713/ab.23.0177
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Objective: The objective of this study was to investigate how cellulase or/and lactic acid bacteria (LAB) affected the fermentation characteristic and microbial community in wet brewer's grains (WBG) and corn stover (CS) mixed silage. Methods: The WBG was mixed thoroughly with the CS at 7:3 (w/w). Four treatment groups were studied: i) CON, no additives; ii) CEL, added cellulase (120 U/g fresh matter [FM]), iii) LAB, added LAB (2x10(6) cfu/g FM), and iv) CLA, added cellulase (120 U/g FM) and LAB (2x10(6 )cfu/g FM). Results: All additive-treated groups showed higher fermentation quality over the 30 d ensiling period. As these groups exhibited higher (p<0.05) LAB counts and lactic acid (LA) content, along with lower pH value and ammonia-nitrogen (NH3-N) content than the control. Specifically, cellulase-treated groups (CEL and CLA) showed lower (p<0.05) neutral detergent fiber and acid detergent fiber contents than other groups. All additives increased the abundance of beneficial bacteria (Firmicutes, Lactiplantibacillus, and Limosilactobacillus) while they decreased abundance of Proteobacteria and microbial diversity as well. Conclusion: The combined application of cellulase and LAB could effectively improve the fermentation quality and microbial community of the WBG and CS mixed silage.
引用
收藏
页码:84 / 94
页数:11
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