Optimization on crystallization process of the magnetic core lysozyme

The continuous growth of the biomedical market highlights the significance of improvement in the efficiency of batch production for protein drug crystals. Herein, we investigated the effect of polymer modified Fe3O4 nanoparticles on lysozyme (Lys) crystallization at different conditions, using functionalized Fe3O4 magnetic nanoparticles (Polymer C@Fe3O4) as nucleation agents. The study revealed that the surface charge density of Polymer C@Fe3O4 was highest at a modification mass ratio of Fe3O4 to Polymer C is 8:3. This ratio facilitates the augmentation of electrostatic interaction and the promotion of crystallization. By adding 15% of the PolymerC@Fe3O4 prepared from Fe3O4 with an average particle size of 150 nm, the magnetic core lysozyme (M-Lys) crystals with an average particle size of 11.08 mm, narrow size distribution, and regular morphology were obtained in a yield of 82.42% within 60 min.& COPY; 2023 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.